Antiadhesive Polymer Brush Coating Functionalized with Antimicrobial and RGD Peptides to Reduce Biofilm Formation and Enhance Tissue Integration

This paper describes the synthesis and characterization of polymer–peptide conjugates to be used as infection-resistant coating for biomaterial implants and devices. Antiadhesive polymer brushes composed of block copolymer Pluronic F-127 (PF127) were functionalized with antimicrobial peptides (AMP),...

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Bibliographic Details
Published in:Biomacromolecules 2014-06, Vol.15 (6), p.2019-2026
Main Authors: Muszanska, Agnieszka K, Rochford, Edward T. J, Gruszka, Agnieszka, Bastian, Andreas A, Busscher, Henk J, Norde, Willem, van der Mei, Henny C, Herrmann, Andreas
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Anti-Infective Agents - chemistry
Anti-Infective Agents - pharmacology
Applied sciences
Bacterial Adhesion - drug effects
Bacterial Adhesion - genetics
Biofilms - drug effects
Biofilms - growth & development
Biological and medical sciences
Chemical modifications
Chemical reactions and properties
Exact sciences and technology
Fibroblasts - drug effects
Fibroblasts - metabolism
Humans
Medical sciences
Molecular Sequence Data
Oligopeptides - chemistry
Oligopeptides - genetics
Oligopeptides - pharmacology
Organic polymers
Physicochemistry of polymers
Polymers - chemistry
Polymers - pharmacology
Pseudomonas aeruginosa
Pseudomonas aeruginosa - drug effects
Pseudomonas aeruginosa - physiology
Staphylococcus aureus
Staphylococcus aureus - drug effects
Staphylococcus aureus - physiology
Staphylococcus epidermidis
Staphylococcus epidermidis - drug effects
Staphylococcus epidermidis - physiology
Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases
Technology. Biomaterials. Equipments
Tissue Distribution - drug effects
Tissue Distribution - physiology
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Summary:This paper describes the synthesis and characterization of polymer–peptide conjugates to be used as infection-resistant coating for biomaterial implants and devices. Antiadhesive polymer brushes composed of block copolymer Pluronic F-127 (PF127) were functionalized with antimicrobial peptides (AMP), able to kill bacteria on contact, and arginine–glycine–aspartate (RGD) peptides to promote the adhesion and spreading of host tissue cells. The antiadhesive and antibacterial properties of the coating were investigated with three bacterial strains: Staphylococcus aureus, Staphylococcus epidermidis, and Pseudomonas aeruginosa. The ability of the coating to support mammalian cell growth was determined using human fibroblast cells. Coatings composed of the appropriate ratio of the functional components: PF127, PF127 modified with AMP, and PF127 modified with RGD showed good antiadhesive and bactericidal properties without hampering tissue compatibility.
ISSN:1525-7797
1526-4602
DOI:10.1021/bm500168s