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Antiadhesive Polymer Brush Coating Functionalized with Antimicrobial and RGD Peptides to Reduce Biofilm Formation and Enhance Tissue Integration
This paper describes the synthesis and characterization of polymer–peptide conjugates to be used as infection-resistant coating for biomaterial implants and devices. Antiadhesive polymer brushes composed of block copolymer Pluronic F-127 (PF127) were functionalized with antimicrobial peptides (AMP),...
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| Published in: | Biomacromolecules 2014-06, Vol.15 (6), p.2019-2026 |
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| Main Authors: | , , , , , , , |
| Format: | Article |
| Language: | English |
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| cited_by | cdi_FETCH-LOGICAL-a378t-ebf928dc73d1dc1299a13145b64055d3813bb2d429b9220d43866f4851e272803 |
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| cites | cdi_FETCH-LOGICAL-a378t-ebf928dc73d1dc1299a13145b64055d3813bb2d429b9220d43866f4851e272803 |
| container_end_page | 2026 |
| container_issue | 6 |
| container_start_page | 2019 |
| container_title | Biomacromolecules |
| container_volume | 15 |
| creator | Muszanska, Agnieszka K Rochford, Edward T. J Gruszka, Agnieszka Bastian, Andreas A Busscher, Henk J Norde, Willem van der Mei, Henny C Herrmann, Andreas |
| description | This paper describes the synthesis and characterization of polymer–peptide conjugates to be used as infection-resistant coating for biomaterial implants and devices. Antiadhesive polymer brushes composed of block copolymer Pluronic F-127 (PF127) were functionalized with antimicrobial peptides (AMP), able to kill bacteria on contact, and arginine–glycine–aspartate (RGD) peptides to promote the adhesion and spreading of host tissue cells. The antiadhesive and antibacterial properties of the coating were investigated with three bacterial strains: Staphylococcus aureus, Staphylococcus epidermidis, and Pseudomonas aeruginosa. The ability of the coating to support mammalian cell growth was determined using human fibroblast cells. Coatings composed of the appropriate ratio of the functional components: PF127, PF127 modified with AMP, and PF127 modified with RGD showed good antiadhesive and bactericidal properties without hampering tissue compatibility. |
| doi_str_mv | 10.1021/bm500168s |
| format | article |
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J ; Gruszka, Agnieszka ; Bastian, Andreas A ; Busscher, Henk J ; Norde, Willem ; van der Mei, Henny C ; Herrmann, Andreas</creator><creatorcontrib>Muszanska, Agnieszka K ; Rochford, Edward T. J ; Gruszka, Agnieszka ; Bastian, Andreas A ; Busscher, Henk J ; Norde, Willem ; van der Mei, Henny C ; Herrmann, Andreas</creatorcontrib><description>This paper describes the synthesis and characterization of polymer–peptide conjugates to be used as infection-resistant coating for biomaterial implants and devices. Antiadhesive polymer brushes composed of block copolymer Pluronic F-127 (PF127) were functionalized with antimicrobial peptides (AMP), able to kill bacteria on contact, and arginine–glycine–aspartate (RGD) peptides to promote the adhesion and spreading of host tissue cells. The antiadhesive and antibacterial properties of the coating were investigated with three bacterial strains: Staphylococcus aureus, Staphylococcus epidermidis, and Pseudomonas aeruginosa. The ability of the coating to support mammalian cell growth was determined using human fibroblast cells. Coatings composed of the appropriate ratio of the functional components: PF127, PF127 modified with AMP, and PF127 modified with RGD showed good antiadhesive and bactericidal properties without hampering tissue compatibility.</description><identifier>ISSN: 1525-7797</identifier><identifier>EISSN: 1526-4602</identifier><identifier>DOI: 10.1021/bm500168s</identifier><identifier>PMID: 24833130</identifier><language>eng</language><publisher>Washington, DC: American Chemical Society</publisher><subject>Amino Acid Sequence ; Anti-Infective Agents - chemistry ; Anti-Infective Agents - pharmacology ; Applied sciences ; Bacterial Adhesion - drug effects ; Bacterial Adhesion - genetics ; Biofilms - drug effects ; Biofilms - growth & development ; Biological and medical sciences ; Chemical modifications ; Chemical reactions and properties ; Exact sciences and technology ; Fibroblasts - drug effects ; Fibroblasts - metabolism ; Humans ; Medical sciences ; Molecular Sequence Data ; Oligopeptides - chemistry ; Oligopeptides - genetics ; Oligopeptides - pharmacology ; Organic polymers ; Physicochemistry of polymers ; Polymers - chemistry ; Polymers - pharmacology ; Pseudomonas aeruginosa ; Pseudomonas aeruginosa - drug effects ; Pseudomonas aeruginosa - physiology ; Staphylococcus aureus ; Staphylococcus aureus - drug effects ; Staphylococcus aureus - physiology ; Staphylococcus epidermidis ; Staphylococcus epidermidis - drug effects ; Staphylococcus epidermidis - physiology ; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases ; Technology. Biomaterials. Equipments ; Tissue Distribution - drug effects ; Tissue Distribution - physiology</subject><ispartof>Biomacromolecules, 2014-06, Vol.15 (6), p.2019-2026</ispartof><rights>Copyright © 2014 American Chemical Society</rights><rights>2015 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a378t-ebf928dc73d1dc1299a13145b64055d3813bb2d429b9220d43866f4851e272803</citedby><cites>FETCH-LOGICAL-a378t-ebf928dc73d1dc1299a13145b64055d3813bb2d429b9220d43866f4851e272803</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=28605208$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24833130$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Muszanska, Agnieszka K</creatorcontrib><creatorcontrib>Rochford, Edward T. J</creatorcontrib><creatorcontrib>Gruszka, Agnieszka</creatorcontrib><creatorcontrib>Bastian, Andreas A</creatorcontrib><creatorcontrib>Busscher, Henk J</creatorcontrib><creatorcontrib>Norde, Willem</creatorcontrib><creatorcontrib>van der Mei, Henny C</creatorcontrib><creatorcontrib>Herrmann, Andreas</creatorcontrib><title>Antiadhesive Polymer Brush Coating Functionalized with Antimicrobial and RGD Peptides to Reduce Biofilm Formation and Enhance Tissue Integration</title><title>Biomacromolecules</title><addtitle>Biomacromolecules</addtitle><description>This paper describes the synthesis and characterization of polymer–peptide conjugates to be used as infection-resistant coating for biomaterial implants and devices. Antiadhesive polymer brushes composed of block copolymer Pluronic F-127 (PF127) were functionalized with antimicrobial peptides (AMP), able to kill bacteria on contact, and arginine–glycine–aspartate (RGD) peptides to promote the adhesion and spreading of host tissue cells. The antiadhesive and antibacterial properties of the coating were investigated with three bacterial strains: Staphylococcus aureus, Staphylococcus epidermidis, and Pseudomonas aeruginosa. The ability of the coating to support mammalian cell growth was determined using human fibroblast cells. Coatings composed of the appropriate ratio of the functional components: PF127, PF127 modified with AMP, and PF127 modified with RGD showed good antiadhesive and bactericidal properties without hampering tissue compatibility.</description><subject>Amino Acid Sequence</subject><subject>Anti-Infective Agents - chemistry</subject><subject>Anti-Infective Agents - pharmacology</subject><subject>Applied sciences</subject><subject>Bacterial Adhesion - drug effects</subject><subject>Bacterial Adhesion - genetics</subject><subject>Biofilms - drug effects</subject><subject>Biofilms - growth & development</subject><subject>Biological and medical sciences</subject><subject>Chemical modifications</subject><subject>Chemical reactions and properties</subject><subject>Exact sciences and technology</subject><subject>Fibroblasts - drug effects</subject><subject>Fibroblasts - metabolism</subject><subject>Humans</subject><subject>Medical sciences</subject><subject>Molecular Sequence Data</subject><subject>Oligopeptides - chemistry</subject><subject>Oligopeptides - genetics</subject><subject>Oligopeptides - pharmacology</subject><subject>Organic polymers</subject><subject>Physicochemistry of polymers</subject><subject>Polymers - chemistry</subject><subject>Polymers - pharmacology</subject><subject>Pseudomonas aeruginosa</subject><subject>Pseudomonas aeruginosa - drug effects</subject><subject>Pseudomonas aeruginosa - physiology</subject><subject>Staphylococcus aureus</subject><subject>Staphylococcus aureus - drug effects</subject><subject>Staphylococcus aureus - physiology</subject><subject>Staphylococcus epidermidis</subject><subject>Staphylococcus epidermidis - drug effects</subject><subject>Staphylococcus epidermidis - physiology</subject><subject>Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases</subject><subject>Technology. Biomaterials. Equipments</subject><subject>Tissue Distribution - drug effects</subject><subject>Tissue Distribution - physiology</subject><issn>1525-7797</issn><issn>1526-4602</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><recordid>eNqN0c1O3DAUBWCralUoZdEXqLyp1C7S-jdxljBlKBISCME6cuIbxiixp74OFX2KPnIzwxQ2LLqypfv5WLqHkA-cfeVM8G_tqBnjpcFXZJ9rURaqZOL19q6LqqqrPfIO8Y4xVkul35I9oYyUXLJ98ucoZG_dCtDfA72Mw8MIiR6nCVd0EW324ZYup9BlH4Md_G9w9JfPK7p5Nvouxdbbgdrg6NXpd3oJ6-wdIM2RXoGbOqDHPvZ-GOkyptFuUrb4JKxsmKfXHnECehYy3Kbt-D1509sB4XB3HpCb5cn14kdxfnF6tjg6L6ysTC6g7WthXFdJx13HRV1bLrnSbamY1k4aLttWOCXqthaCOSVNWfbKaA6iEobJA_L5MXed4s8JMDejxw6GwQaIEzZcK8X4nMn_g0qlKiGlnumXRzpvBjFB36yTH216aDhrNl01T13N9uMudmpHcE_yXzkz-LQDFjs79GlemcdnZ0qmBTPPznbY3MUpzU3hCx_-BZ5ap6o</recordid><startdate>20140609</startdate><enddate>20140609</enddate><creator>Muszanska, Agnieszka K</creator><creator>Rochford, Edward T. 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Antiadhesive polymer brushes composed of block copolymer Pluronic F-127 (PF127) were functionalized with antimicrobial peptides (AMP), able to kill bacteria on contact, and arginine–glycine–aspartate (RGD) peptides to promote the adhesion and spreading of host tissue cells. The antiadhesive and antibacterial properties of the coating were investigated with three bacterial strains: Staphylococcus aureus, Staphylococcus epidermidis, and Pseudomonas aeruginosa. The ability of the coating to support mammalian cell growth was determined using human fibroblast cells. Coatings composed of the appropriate ratio of the functional components: PF127, PF127 modified with AMP, and PF127 modified with RGD showed good antiadhesive and bactericidal properties without hampering tissue compatibility.</abstract><cop>Washington, DC</cop><pub>American Chemical Society</pub><pmid>24833130</pmid><doi>10.1021/bm500168s</doi><tpages>8</tpages></addata></record> |
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| ispartof | Biomacromolecules, 2014-06, Vol.15 (6), p.2019-2026 |
| issn | 1525-7797 1526-4602 |
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| source | American Chemical Society:Jisc Collections:American Chemical Society Read & Publish Agreement 2022-2024 (Reading list) |
| subjects | Amino Acid Sequence Anti-Infective Agents - chemistry Anti-Infective Agents - pharmacology Applied sciences Bacterial Adhesion - drug effects Bacterial Adhesion - genetics Biofilms - drug effects Biofilms - growth & development Biological and medical sciences Chemical modifications Chemical reactions and properties Exact sciences and technology Fibroblasts - drug effects Fibroblasts - metabolism Humans Medical sciences Molecular Sequence Data Oligopeptides - chemistry Oligopeptides - genetics Oligopeptides - pharmacology Organic polymers Physicochemistry of polymers Polymers - chemistry Polymers - pharmacology Pseudomonas aeruginosa Pseudomonas aeruginosa - drug effects Pseudomonas aeruginosa - physiology Staphylococcus aureus Staphylococcus aureus - drug effects Staphylococcus aureus - physiology Staphylococcus epidermidis Staphylococcus epidermidis - drug effects Staphylococcus epidermidis - physiology Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases Technology. Biomaterials. Equipments Tissue Distribution - drug effects Tissue Distribution - physiology |
| title | Antiadhesive Polymer Brush Coating Functionalized with Antimicrobial and RGD Peptides to Reduce Biofilm Formation and Enhance Tissue Integration |
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