Surface modification and evaluation of PLGA nanoparticles: the effects on cellular uptake and cell proliferation on the HT-29 cell line

The main objectives of the present research were to determine the effects of different coating agents and techniques on different characteristics, and cellular uptake of a new antiproliferative drug meloxicam loaded PLGA nanoparticles, and to evaluate their safety and potential use as drug carriers...

Full description

Saved in:
Bibliographic Details
Published in:Journal of drug delivery science and technology 2014-01, Vol.24 (2), p.166-172
Main Authors: Sengel-Turk, C.T., Hascicek, C., Dogan, A.L., Esendagli, G., Guc, D., Gonul, N.
Format: Article
Language:English
Subjects:
Cellular uptake
Didodecyldimethylammonium bromide
ethylene glycol 2000
glycolide) nanoparticles
lactide
Meloxicam
Poly
Poly(D,L
Surface coating
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The main objectives of the present research were to determine the effects of different coating agents and techniques on different characteristics, and cellular uptake of a new antiproliferative drug meloxicam loaded PLGA nanoparticles, and to evaluate their safety and potential use as drug carriers for colon cancer treatment. For this purpose, nanoparticles with a mean diameter of 178.30 nm were successfully prepared. Two different surface coating techniques the in situ coating technique and the surface adsorption method were used as coating processes with PEG 2000 and DMAB. A rate of 2.5 % DMAB-coated PLGA nanoparticles had an average size of 177.50 nm with a zeta potential of approximately + 4.72 mV, while PEG-modified nanoparticles using the in situ coating technique had a smaller particle size and negative zeta potential. The cell viability assays demonstrated that the cytotoxicity of nanoparticles was significantly affected by the coating process and both of the modified formulations were found more potent than pure meloxicam.
ISSN:1773-2247
DOI:10.1016/S1773-2247(14)50027-5