Proteomics screening of molecular targets of curcumin in mouse brain

Curcumin is one of the most important constituent of Curcuma longa L. with antioxidant, anti-inflammatory and anticancer effects. In this study, we investigated potential intracellular targets of curcumin by affinity chromatography based on target deconvolution. Identification of curcumin interactin...

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Bibliographic Details
Published in:Life sciences (1973) 2014-03, Vol.98 (1), p.12-17
Main Authors: Firouzi, Zohreh, Lari, Parisa, Rashedinia, Marzieh, Ramezani, Mohammad, Iranshahi, Mehrdad, Abnous, Khalil
Format: Article
Language:English
Subjects:
Affinity chromatography
Amino Acid Sequence
Animals
Anti-Inflammatory Agents - pharmacology
Brain - drug effects
Curcuma longa L
Curcumin
Curcumin - pharmacology
Drug Delivery Systems
Electrophoresis, Gel, Two-Dimensional
Gene Expression Regulation - drug effects
Heat-Shock Proteins - genetics
Male
Mice
Mice, Inbred BALB C
Molecular Sequence Data
Proteins - genetics
Proteomics
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Target deconvolution
Tubulin - genetics
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Summary:Curcumin is one of the most important constituent of Curcuma longa L. with antioxidant, anti-inflammatory and anticancer effects. In this study, we investigated potential intracellular targets of curcumin by affinity chromatography based on target deconvolution. Identification of curcumin interacting proteins may help in evaluating biological and side effects of this natural compound. Curcumin was immobilized through a linker to sepharose beads as solid matrix. Pull down assay was performed by passing tissue lysate of mouse brain through the column to enrich and purify curcumin interacting proteins. Then proteins were separated using two-dimensional gel electrophoresis and identified using MALDI/TOF/TOF mass spectrometry. Our results show that curcumin physically binds to a wide range of cellular proteins including structural proteins, metabolic enzymes and proteins involved in apoptosis pathway. Finding curcumin interacting proteins may help in understanding a part of curcumin pharmacological effects.
ISSN:0024-3205
1879-0631
DOI:10.1016/j.lfs.2013.12.200