Loading…
A proteomic approach of pediatric astrocytomas: MiRNAs and network insight
Pediatric astrocytomas, a leading cause of death associated with cancer, are the most common primary central nervous system tumors found in children. Most studies of these tumors focus on adults, not on children. We examined the global protein and microRNA expression pattern by 2D SDS-PAGE, mass spe...
Saved in:
Published in: | Journal of proteomics 2013-12, Vol.94, p.162-175 |
---|---|
Main Authors: | , , , , , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
cited_by | cdi_FETCH-LOGICAL-c416t-541ceb4af4a5f36e039bc9e5a641801366d786f58795b55961f7a3339a0d74ad3 |
---|---|
cites | cdi_FETCH-LOGICAL-c416t-541ceb4af4a5f36e039bc9e5a641801366d786f58795b55961f7a3339a0d74ad3 |
container_end_page | 175 |
container_issue | |
container_start_page | 162 |
container_title | Journal of proteomics |
container_volume | 94 |
creator | Ruiz Esparza-Garrido, Ruth Velázquez-Flores, Miguel Á. Diegopérez-Ramírez, Jaime López-Aguilar, Enrique Siordia-Reyes, Georgina Hernández-Ortiz, Magdalena Martínez-Batallar, Ángel G. Encarnación-Guevara, Sergio Salamanca-Gómez, Fabio Arenas-Aranda, Diego Julio |
description | Pediatric astrocytomas, a leading cause of death associated with cancer, are the most common primary central nervous system tumors found in children. Most studies of these tumors focus on adults, not on children. We examined the global protein and microRNA expression pattern by 2D SDS-PAGE, mass spectrometry (MALDI-TOF), and RT2 miRNA PCR Array System. Proteomic studies revealed 49 proteins with changes on the expression. Interactome showed that vimentin, calreticulin, and 14-3-3 epsilon protein are hub proteins in these neoplasms. MicroRNA analyses demonstrated for the first time novel microRNAs involved in the astrocytoma biology. In conclusion, our results show that novel proteins and microRNAs with expression changes on pediatric astrocytoma could serve as biomarkers of tumor progression.
Astrocytomas are tumors that progress rapidly and that invade surrounding tissues. Although some drugs have been developed to treat these neoplasms, the mortality of patients is still very high. In this study, we describe for the first time, to our knowledge, some proteins and miRNAs associated with the biology of astrocytic tumors that could be postulated as possible diagnostic or prognostic biomarkers. Altogether, our results indicate that large-scale analyses allow making a fairly accurate prediction of different cellular processes altered in astrocytic tumors.
[Display omitted]
•Forty nine proteins with expression changes in pediatric astrocytomas are reported.•Calreticulin, vimentin, and 1433 epsilon protein are possible hub proteins.•Novel microRNAs, involved in astrocytoma biology, are revealed for the first time.•Altered proteins and microRNAs are involved in the same signaling pathways.•Some protein changes appear to correlate with microRNA alteration. |
doi_str_mv | 10.1016/j.jprot.2013.09.009 |
format | article |
fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1544017611</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S1874391913004752</els_id><sourcerecordid>1544017611</sourcerecordid><originalsourceid>FETCH-LOGICAL-c416t-541ceb4af4a5f36e039bc9e5a641801366d786f58795b55961f7a3339a0d74ad3</originalsourceid><addsrcrecordid>eNp9kLtOwzAUQD2ARCl8AQMZWRLs-hUjMVQVTxWQgM6W6zitQxMX2wX173EIM9O9ujr3dQA4Q7BAELHLpmi23sViAhEuoCggFAdghEpOciyQOALHITQQMsQFH4HHadbTxrVWZ2qbcqXXmauzramsir6vhuid3kfXqnCVPdnX52nIVFdlnYnfzn9ktgt2tY4n4LBWm2BO_-IYLG5v3mf3-fzl7mE2neeaIBZzSpA2S6JqomiNmYFYLLUwVDGCynQzYxUvWU1LLuiSUsFQzRXGWChYcaIqPAYXw9x07OfOhChbG7TZbFRn3C5IRAmBiDOEEooHVHsXgje13HrbKr-XCMrelmzkry3Z25JQyGQrdZ0PXbVyUq28DXLxlgAKIcR8UvZzrwfCpD-_rPEyaGs6nZx5o6OsnP13ww9mb38p</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1544017611</pqid></control><display><type>article</type><title>A proteomic approach of pediatric astrocytomas: MiRNAs and network insight</title><source>ScienceDirect Freedom Collection 2022-2024</source><creator>Ruiz Esparza-Garrido, Ruth ; Velázquez-Flores, Miguel Á. ; Diegopérez-Ramírez, Jaime ; López-Aguilar, Enrique ; Siordia-Reyes, Georgina ; Hernández-Ortiz, Magdalena ; Martínez-Batallar, Ángel G. ; Encarnación-Guevara, Sergio ; Salamanca-Gómez, Fabio ; Arenas-Aranda, Diego Julio</creator><creatorcontrib>Ruiz Esparza-Garrido, Ruth ; Velázquez-Flores, Miguel Á. ; Diegopérez-Ramírez, Jaime ; López-Aguilar, Enrique ; Siordia-Reyes, Georgina ; Hernández-Ortiz, Magdalena ; Martínez-Batallar, Ángel G. ; Encarnación-Guevara, Sergio ; Salamanca-Gómez, Fabio ; Arenas-Aranda, Diego Julio</creatorcontrib><description>Pediatric astrocytomas, a leading cause of death associated with cancer, are the most common primary central nervous system tumors found in children. Most studies of these tumors focus on adults, not on children. We examined the global protein and microRNA expression pattern by 2D SDS-PAGE, mass spectrometry (MALDI-TOF), and RT2 miRNA PCR Array System. Proteomic studies revealed 49 proteins with changes on the expression. Interactome showed that vimentin, calreticulin, and 14-3-3 epsilon protein are hub proteins in these neoplasms. MicroRNA analyses demonstrated for the first time novel microRNAs involved in the astrocytoma biology. In conclusion, our results show that novel proteins and microRNAs with expression changes on pediatric astrocytoma could serve as biomarkers of tumor progression.
Astrocytomas are tumors that progress rapidly and that invade surrounding tissues. Although some drugs have been developed to treat these neoplasms, the mortality of patients is still very high. In this study, we describe for the first time, to our knowledge, some proteins and miRNAs associated with the biology of astrocytic tumors that could be postulated as possible diagnostic or prognostic biomarkers. Altogether, our results indicate that large-scale analyses allow making a fairly accurate prediction of different cellular processes altered in astrocytic tumors.
[Display omitted]
•Forty nine proteins with expression changes in pediatric astrocytomas are reported.•Calreticulin, vimentin, and 1433 epsilon protein are possible hub proteins.•Novel microRNAs, involved in astrocytoma biology, are revealed for the first time.•Altered proteins and microRNAs are involved in the same signaling pathways.•Some protein changes appear to correlate with microRNA alteration.</description><identifier>ISSN: 1874-3919</identifier><identifier>DOI: 10.1016/j.jprot.2013.09.009</identifier><language>eng</language><publisher>Elsevier B.V</publisher><subject>adults ; biomarkers ; calreticulin ; central nervous system ; children ; death ; drugs ; Hub proteins ; mass spectrometry ; microRNA ; mortality ; neoplasms ; Novel miRNAs ; patients ; Pediatric astrocytoma ; polyacrylamide gel electrophoresis ; polymerase chain reaction ; prediction ; Protein–protein interactions ; Proteomic ; proteomics ; vimentin</subject><ispartof>Journal of proteomics, 2013-12, Vol.94, p.162-175</ispartof><rights>2013 Elsevier B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c416t-541ceb4af4a5f36e039bc9e5a641801366d786f58795b55961f7a3339a0d74ad3</citedby><cites>FETCH-LOGICAL-c416t-541ceb4af4a5f36e039bc9e5a641801366d786f58795b55961f7a3339a0d74ad3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids></links><search><creatorcontrib>Ruiz Esparza-Garrido, Ruth</creatorcontrib><creatorcontrib>Velázquez-Flores, Miguel Á.</creatorcontrib><creatorcontrib>Diegopérez-Ramírez, Jaime</creatorcontrib><creatorcontrib>López-Aguilar, Enrique</creatorcontrib><creatorcontrib>Siordia-Reyes, Georgina</creatorcontrib><creatorcontrib>Hernández-Ortiz, Magdalena</creatorcontrib><creatorcontrib>Martínez-Batallar, Ángel G.</creatorcontrib><creatorcontrib>Encarnación-Guevara, Sergio</creatorcontrib><creatorcontrib>Salamanca-Gómez, Fabio</creatorcontrib><creatorcontrib>Arenas-Aranda, Diego Julio</creatorcontrib><title>A proteomic approach of pediatric astrocytomas: MiRNAs and network insight</title><title>Journal of proteomics</title><description>Pediatric astrocytomas, a leading cause of death associated with cancer, are the most common primary central nervous system tumors found in children. Most studies of these tumors focus on adults, not on children. We examined the global protein and microRNA expression pattern by 2D SDS-PAGE, mass spectrometry (MALDI-TOF), and RT2 miRNA PCR Array System. Proteomic studies revealed 49 proteins with changes on the expression. Interactome showed that vimentin, calreticulin, and 14-3-3 epsilon protein are hub proteins in these neoplasms. MicroRNA analyses demonstrated for the first time novel microRNAs involved in the astrocytoma biology. In conclusion, our results show that novel proteins and microRNAs with expression changes on pediatric astrocytoma could serve as biomarkers of tumor progression.
Astrocytomas are tumors that progress rapidly and that invade surrounding tissues. Although some drugs have been developed to treat these neoplasms, the mortality of patients is still very high. In this study, we describe for the first time, to our knowledge, some proteins and miRNAs associated with the biology of astrocytic tumors that could be postulated as possible diagnostic or prognostic biomarkers. Altogether, our results indicate that large-scale analyses allow making a fairly accurate prediction of different cellular processes altered in astrocytic tumors.
[Display omitted]
•Forty nine proteins with expression changes in pediatric astrocytomas are reported.•Calreticulin, vimentin, and 1433 epsilon protein are possible hub proteins.•Novel microRNAs, involved in astrocytoma biology, are revealed for the first time.•Altered proteins and microRNAs are involved in the same signaling pathways.•Some protein changes appear to correlate with microRNA alteration.</description><subject>adults</subject><subject>biomarkers</subject><subject>calreticulin</subject><subject>central nervous system</subject><subject>children</subject><subject>death</subject><subject>drugs</subject><subject>Hub proteins</subject><subject>mass spectrometry</subject><subject>microRNA</subject><subject>mortality</subject><subject>neoplasms</subject><subject>Novel miRNAs</subject><subject>patients</subject><subject>Pediatric astrocytoma</subject><subject>polyacrylamide gel electrophoresis</subject><subject>polymerase chain reaction</subject><subject>prediction</subject><subject>Protein–protein interactions</subject><subject>Proteomic</subject><subject>proteomics</subject><subject>vimentin</subject><issn>1874-3919</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><recordid>eNp9kLtOwzAUQD2ARCl8AQMZWRLs-hUjMVQVTxWQgM6W6zitQxMX2wX173EIM9O9ujr3dQA4Q7BAELHLpmi23sViAhEuoCggFAdghEpOciyQOALHITQQMsQFH4HHadbTxrVWZ2qbcqXXmauzramsir6vhuid3kfXqnCVPdnX52nIVFdlnYnfzn9ktgt2tY4n4LBWm2BO_-IYLG5v3mf3-fzl7mE2neeaIBZzSpA2S6JqomiNmYFYLLUwVDGCynQzYxUvWU1LLuiSUsFQzRXGWChYcaIqPAYXw9x07OfOhChbG7TZbFRn3C5IRAmBiDOEEooHVHsXgje13HrbKr-XCMrelmzkry3Z25JQyGQrdZ0PXbVyUq28DXLxlgAKIcR8UvZzrwfCpD-_rPEyaGs6nZx5o6OsnP13ww9mb38p</recordid><startdate>20131206</startdate><enddate>20131206</enddate><creator>Ruiz Esparza-Garrido, Ruth</creator><creator>Velázquez-Flores, Miguel Á.</creator><creator>Diegopérez-Ramírez, Jaime</creator><creator>López-Aguilar, Enrique</creator><creator>Siordia-Reyes, Georgina</creator><creator>Hernández-Ortiz, Magdalena</creator><creator>Martínez-Batallar, Ángel G.</creator><creator>Encarnación-Guevara, Sergio</creator><creator>Salamanca-Gómez, Fabio</creator><creator>Arenas-Aranda, Diego Julio</creator><general>Elsevier B.V</general><scope>FBQ</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope></search><sort><creationdate>20131206</creationdate><title>A proteomic approach of pediatric astrocytomas: MiRNAs and network insight</title><author>Ruiz Esparza-Garrido, Ruth ; Velázquez-Flores, Miguel Á. ; Diegopérez-Ramírez, Jaime ; López-Aguilar, Enrique ; Siordia-Reyes, Georgina ; Hernández-Ortiz, Magdalena ; Martínez-Batallar, Ángel G. ; Encarnación-Guevara, Sergio ; Salamanca-Gómez, Fabio ; Arenas-Aranda, Diego Julio</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c416t-541ceb4af4a5f36e039bc9e5a641801366d786f58795b55961f7a3339a0d74ad3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>adults</topic><topic>biomarkers</topic><topic>calreticulin</topic><topic>central nervous system</topic><topic>children</topic><topic>death</topic><topic>drugs</topic><topic>Hub proteins</topic><topic>mass spectrometry</topic><topic>microRNA</topic><topic>mortality</topic><topic>neoplasms</topic><topic>Novel miRNAs</topic><topic>patients</topic><topic>Pediatric astrocytoma</topic><topic>polyacrylamide gel electrophoresis</topic><topic>polymerase chain reaction</topic><topic>prediction</topic><topic>Protein–protein interactions</topic><topic>Proteomic</topic><topic>proteomics</topic><topic>vimentin</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ruiz Esparza-Garrido, Ruth</creatorcontrib><creatorcontrib>Velázquez-Flores, Miguel Á.</creatorcontrib><creatorcontrib>Diegopérez-Ramírez, Jaime</creatorcontrib><creatorcontrib>López-Aguilar, Enrique</creatorcontrib><creatorcontrib>Siordia-Reyes, Georgina</creatorcontrib><creatorcontrib>Hernández-Ortiz, Magdalena</creatorcontrib><creatorcontrib>Martínez-Batallar, Ángel G.</creatorcontrib><creatorcontrib>Encarnación-Guevara, Sergio</creatorcontrib><creatorcontrib>Salamanca-Gómez, Fabio</creatorcontrib><creatorcontrib>Arenas-Aranda, Diego Julio</creatorcontrib><collection>AGRIS</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><jtitle>Journal of proteomics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ruiz Esparza-Garrido, Ruth</au><au>Velázquez-Flores, Miguel Á.</au><au>Diegopérez-Ramírez, Jaime</au><au>López-Aguilar, Enrique</au><au>Siordia-Reyes, Georgina</au><au>Hernández-Ortiz, Magdalena</au><au>Martínez-Batallar, Ángel G.</au><au>Encarnación-Guevara, Sergio</au><au>Salamanca-Gómez, Fabio</au><au>Arenas-Aranda, Diego Julio</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A proteomic approach of pediatric astrocytomas: MiRNAs and network insight</atitle><jtitle>Journal of proteomics</jtitle><date>2013-12-06</date><risdate>2013</risdate><volume>94</volume><spage>162</spage><epage>175</epage><pages>162-175</pages><issn>1874-3919</issn><abstract>Pediatric astrocytomas, a leading cause of death associated with cancer, are the most common primary central nervous system tumors found in children. Most studies of these tumors focus on adults, not on children. We examined the global protein and microRNA expression pattern by 2D SDS-PAGE, mass spectrometry (MALDI-TOF), and RT2 miRNA PCR Array System. Proteomic studies revealed 49 proteins with changes on the expression. Interactome showed that vimentin, calreticulin, and 14-3-3 epsilon protein are hub proteins in these neoplasms. MicroRNA analyses demonstrated for the first time novel microRNAs involved in the astrocytoma biology. In conclusion, our results show that novel proteins and microRNAs with expression changes on pediatric astrocytoma could serve as biomarkers of tumor progression.
Astrocytomas are tumors that progress rapidly and that invade surrounding tissues. Although some drugs have been developed to treat these neoplasms, the mortality of patients is still very high. In this study, we describe for the first time, to our knowledge, some proteins and miRNAs associated with the biology of astrocytic tumors that could be postulated as possible diagnostic or prognostic biomarkers. Altogether, our results indicate that large-scale analyses allow making a fairly accurate prediction of different cellular processes altered in astrocytic tumors.
[Display omitted]
•Forty nine proteins with expression changes in pediatric astrocytomas are reported.•Calreticulin, vimentin, and 1433 epsilon protein are possible hub proteins.•Novel microRNAs, involved in astrocytoma biology, are revealed for the first time.•Altered proteins and microRNAs are involved in the same signaling pathways.•Some protein changes appear to correlate with microRNA alteration.</abstract><pub>Elsevier B.V</pub><doi>10.1016/j.jprot.2013.09.009</doi><tpages>14</tpages></addata></record> |
fulltext | fulltext |
identifier | ISSN: 1874-3919 |
ispartof | Journal of proteomics, 2013-12, Vol.94, p.162-175 |
issn | 1874-3919 |
language | eng |
recordid | cdi_proquest_miscellaneous_1544017611 |
source | ScienceDirect Freedom Collection 2022-2024 |
subjects | adults biomarkers calreticulin central nervous system children death drugs Hub proteins mass spectrometry microRNA mortality neoplasms Novel miRNAs patients Pediatric astrocytoma polyacrylamide gel electrophoresis polymerase chain reaction prediction Protein–protein interactions Proteomic proteomics vimentin |
title | A proteomic approach of pediatric astrocytomas: MiRNAs and network insight |
url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-25T08%3A53%3A39IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=A%20proteomic%20approach%20of%20pediatric%20astrocytomas:%20MiRNAs%20and%20network%20insight&rft.jtitle=Journal%20of%20proteomics&rft.au=Ruiz%20Esparza-Garrido,%20Ruth&rft.date=2013-12-06&rft.volume=94&rft.spage=162&rft.epage=175&rft.pages=162-175&rft.issn=1874-3919&rft_id=info:doi/10.1016/j.jprot.2013.09.009&rft_dat=%3Cproquest_cross%3E1544017611%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c416t-541ceb4af4a5f36e039bc9e5a641801366d786f58795b55961f7a3339a0d74ad3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=1544017611&rft_id=info:pmid/&rfr_iscdi=true |