A Single-Step Extraction for Screening Whole Blood for Basic Drugs by Capillary GC/NPD

Basic drugs were routinely extracted from whole blood under alkaline conditions into n-butyl acetate. An aliquot of the n-butyl acetate was injected into a gas chromatograph equipped with a nitrogen-phosphorous detector and a wide-bore cross-linked 50% phenylmethyl silicone capillary column. Absolut...

Full description

Saved in:
Bibliographic Details
Published in:Journal of analytical toxicology 1989-07, Vol.13 (4), p.224-228
Main Authors: Cox, Robert A., Crifasi, Joseph A., Dickey, Ralph E., Ketzler, Steven C., Pshak, Gary L.
Format: Article
Language:English
Subjects:
Acetic acid
Analysis
Biological and medical sciences
Blood
Chromatography, Gas - methods
Drugs
Forensic science
General pharmacology
Humans
Medical sciences
Pharmaceutical Preparations - blood
Pharmacology. Drug treatments
Silicones
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Basic drugs were routinely extracted from whole blood under alkaline conditions into n-butyl acetate. An aliquot of the n-butyl acetate was injected into a gas chromatograph equipped with a nitrogen-phosphorous detector and a wide-bore cross-linked 50% phenylmethyl silicone capillary column. Absolute and relative retention times were recorded for more than 100 extracted drug standards. Recovery from whole blood was determined for some of the more frequently encountered drugs. This one-step extraction proved to be reliable for general screening and has been used routinely in forensic and clinical toxicological analyses.
ISSN:0146-4760
1945-2403
DOI:10.1093/jat/13.4.224