Evaluation of Vitek 2 for the identification of Candida yeasts

The aim of this investigation was to evaluate the performance of Vitek 2 YST cards (bioMérieux, Inc., Hazelwood, MO, USA) for the identification of yeasts of the genus Candida. A total of 168 isolates were analyzed and the results were compared to those of the API 20 C AUX (24%) o API ID 32 C (76%)...

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Bibliographic Details
Published in:Revista argentina de microbiología 2014-04, Vol.46 (2), p.107-110
Main Authors: Ochiuzzi, María E, Cataldi, Silvana, Guelfand, Liliana, Maldonado, Ivana, Arechavala, Alicia
Format: Article
Language:Spanish
Subjects:
Candida - isolation & purification
Humans
Mycology - instrumentation
Mycology - methods
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Summary:The aim of this investigation was to evaluate the performance of Vitek 2 YST cards (bioMérieux, Inc., Hazelwood, MO, USA) for the identification of yeasts of the genus Candida. A total of 168 isolates were analyzed and the results were compared to those of the API 20 C AUX (24%) o API ID 32 C (76%) kits (bioMérieux, Marcy L'Etoile, France). Each isolate was grown in chromogenic agar and in corn meal agar (Oxoid, UK) to observe its micromorphology. C. albicans and C. dublininesis were identified by additional biochemical and molecular tests. The agreement observed was 98.3%. Only three isolates were incorrectly identified by Vitek 2: one strain of C .tropicalis and one strain of C. krusei were identified as C. parapsilosis by YST while one strain of C. krusei was identified with low discrimination. The average time for obtaining results was 18.25 h. Vitek 2 is a simple, safe and useful system for the identification of significant Candida species.
ISSN:0325-7541
DOI:10.1016/S0325-7541(14)70057-4