Endotoxin Tolerance Drives Neutrophil To Infectious Site

ABSTRACTThe objective of this randomized animal study and laboratory investigation was to investigate whether lipopolysaccharide tolerance redirects neutrophil migration between organs. Male BALB/c mice received subcutaneous injections of lipopolysaccharide (1 mg/kg) for 5 days, followed by cecal li...

Full description

Saved in:
Bibliographic Details
Published in:Shock (Augusta, Ga.) Ga.), 2014-08, Vol.42 (2), p.168-173
Main Authors: Ariga, Suely Kubo, Abatepaulo, Fátima Bernardes, Melo, Edielle Sant Anna, Velasco, Irineu Tadeu, da Silva, Fabiano Pinheiro, de Lima, Thais Martins, Soriano, Francisco Garcia
Format: Article
Language:English
Subjects:
Animals
Cell Adhesion Molecules - metabolism
Cytokines - blood
Immune Tolerance
Lipopolysaccharides - immunology
Lung - immunology
Male
Mice, Inbred BALB C
Neutrophil Infiltration - immunology
Neutrophils - immunology
Peritoneal Cavity - cytology
Sepsis - immunology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:ABSTRACTThe objective of this randomized animal study and laboratory investigation was to investigate whether lipopolysaccharide tolerance redirects neutrophil migration between organs. Male BALB/c mice received subcutaneous injections of lipopolysaccharide (1 mg/kg) for 5 days, followed by cecal ligation and puncture (CLP). Cytokines and adhesion molecules were measured after tolerance and CLP challenge. Increased numbers of neutrophils were observed in the peritoneal cavity of tolerant mice, which was associated with increased levels of adhesion molecules and chemokines. In contrast, nontolerant mice accumulated higher numbers of neutrophils in the lungs compared with those in the peritoneal cavity. Neutrophil function accessed by hydrogen peroxide production from neutrophils recovered from peritoneal cavity showed that tolerance increased the capacity to produce hydrogen peroxide. Mortality was reduced in tolerant animals. This study demonstrated that tolerance reduces leukocyte accumulation in the lung after CLP by redirecting neutrophils to the site of infection.
ISSN:1073-2322
1540-0514
DOI:10.1097/SHK.0000000000000175