Relationships among Gene Expression and Anthocyanin Composition of Malbec Grapevine Clones

Anthocyanin profiles are commonly used for grapevine cultivar identification because it is currently accepted that this trait is closely related to their genetic characteristics. Nevertheless, the extent of the variation for the anthocyanin profiles among clones of the same cultivar has not yet been...

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Bibliographic Details
Published in:Journal of agricultural and food chemistry 2014-07, Vol.62 (28), p.6716-6725
Main Authors: Muñoz, Claudio, Gomez-Talquenca, Sebastian, Chialva, Constanza, Ibáñez, Javier, Martinez-Zapater, José M, Peña-Neira, Álvaro, Lijavetzky, Diego
Format: Article
Language:English
Subjects:
anthocyanins
Anthocyanins - analysis
Clone Cells
clones
cultivar identification
cultivars
Fruit - chemistry
Gene Expression
Gene Expression Profiling
gene expression regulation
Gene Expression Regulation, Plant
Genes, Plant - genetics
high performance liquid chromatography
hydroxylation
malvidin
methylation
Plant Extracts - chemistry
polymerase chain reaction
regulator genes
Species Specificity
vineyards
Vitis
Vitis - chemistry
Vitis - classification
Vitis - genetics
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Summary:Anthocyanin profiles are commonly used for grapevine cultivar identification because it is currently accepted that this trait is closely related to their genetic characteristics. Nevertheless, the extent of the variation for the anthocyanin profiles among clones of the same cultivar has not yet been studied in depth. The relative concentration of anthocyanins of 131 Malbec clones grown in the same vineyard was investigated by HPLC-DAD and the use of comprehensive statistic procedures. Complementarily, the expression level of structural and regulatory genes was studied via real time polymerase chain reaction. Significant variation was identified among the profiles of the clones, mainly due to variations in the amounts of malvidin derivatives. Finally, the differential expression in F3′5′H, OMT1 and AM2 genes seems to be related to the malvidin content variation. This work shows the existence of variation for the anthocyanin profiles among clones from the same grapevine cultivar and the putative involvement of genes related to hydroxylation, methylation, and transport of anthocyanins on the basis of such variation.
ISSN:0021-8561
1520-5118
DOI:10.1021/jf501575m