Predation of the Newly Invasive Pest Megacopta cribraria (Hemiptera: Plataspidae) in Soybean Habitats Adjacent to Cotton by a Complex of Predators

• Published in: Journal of economic entomology 2014-06, Vol.107 (3), p.947-954
• Main Authors: Greenstone, M. H., Tillman, P. G., Hu, J. S.
• Format: Article
• Language: English
• Subjects: Animal Distribution; Animals; Ants - physiology; BIOLOGICAL AND MICROBIAL CONTROL; Biological control; Coleoptera - physiology; Content analysis; Cotton; Crops; Crops, Agricultural - growth & development; Deoxyribonucleic acid; DNA; Electron Transport Complex IV - genetics; Euschistus tristigmus; Food Chain; Geocoris spp; Georgia; Glycine max - growth & development; Gossypium - growth & development; Hemiptera; Heteroptera - genetics; Heteroptera - physiology; Insect Proteins - genetics; Insects; Invasive species; kudzu bug; Megacopta cribraria; Migration; molecular gut-content analysis; Molecular Sequence Data; Oxyopes salticus; Pests; Piezodorus guildinii; Plataspidae; Polymerase Chain Reaction; Predation; Predators; Prey; Sequence Analysis, DNA; Sequence Homology, Nucleic Acid; Soybeans; Spiders - physiology; Thyanta custator
• ISSN: 0022-0493; 1938-291X; 0022-0493
• DOI: 10.1603/EC13356

The kudzu bug, Megacopta cribraria (F.) (Hemiptera: Plataspidae), is a newly invasive exotic insect found primarily on kudzu, but also on soybean, in the southeastern United States. We used molecular gut-content analysis to document predation on this pest by insects and spiders in soybean, and to detect remains of crop-specific alternative prey in predators' guts as markers of predator migration between soybean and adjacent cotton. M. cribraria was found exclusively on soybean. Eight native generalist predators over both crops screened positive by specific PCR for DNA of the pest: Geocoris punctipes (Say), Geocoris uliginosus (Say), Orius insidiosus (Say), Podisus maculiventris (Say), Hippodamia convergens Guérin-Méneville, Zelus renardii (Kolenati), Oxyopes salticus Hentz, and Peucetia viridans (Hentz) ; a ninth predator, the exotic Solenopsis invicta Buren, also screened positive for M. cribraria DNA. P. viridans was the only arthropod that tested positive for DNA of this invasive pest in only one crop, cotton. Two plant-feeding pentatomid species, Piezodorus guildinii (Westwood) and Thyanta custator (F.), were found exclusively on soybean, and another, Euschistus tristigmus (Say), was specific to cotton in the context of this study. Detection of predation on a combination of M. cribraria and P. guildinii and T. custator in cotton and M. cribraria and E. tristigmus in soybean demonstrated that these predators dispersed between crops. These results strongly support the use of soybean habitats adjacent to cotton as part of a conservation biological control strategy against M. cribraria. This is the first report documenting predation on this exotic pest in the field via molecular gut-content analysis.