Tandem mass spectrometry for on-line fermentation monitoring

Membrane introduction mass spectrometry has been employed for on-line determination of the major products and volatile metabolites of Bacillus polymyxa fermentation. Samples were introduced into the mass spectrometer via a direct insertion membrane probe in which the aqueous solution flowed past a m...

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Bibliographic Details
Published in:Biotechnology techniques 1989-11, Vol.3 (6), p.361-364
Main Authors: HAYWARD, M. J, LISTER, A. K, KOTIAHO, T, COOKS, R. G, AUSTIN, G. D, NARAYAN, R, TSAO, G. T
Format: Article
Language:English
Subjects:
2,3-butylene glycol
acetic acid
acetoin
Bacillus polymyxa
Biological and medical sciences
Biotechnology
carbon dioxide
ethanol
fermentation
Fundamental and applied biological sciences. Psychology
Methods. Procedures. Technologies
Microbial engineering. Fermentation and microbial culture technology
oxygen
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Summary:Membrane introduction mass spectrometry has been employed for on-line determination of the major products and volatile metabolites of Bacillus polymyxa fermentation. Samples were introduced into the mass spectrometer via a direct insertion membrane probe in which the aqueous solution flowed past a membrane located in the ion source of the mass spectrometer. Concentrations of the products 2,3-butanediol, acetoin, ethanol and acetic acid in fermentation broth were measured by tandem mass spectrometry after permeation through the membrane and ionization by chemical ionization. Dissolved CO sub(2) and O sub(2), as well as CO sub(2) in the off gases, were also monitored on-line by mass spectrometry. The use of tandem mass spectrometry has allowed the identification of products that were not previously known to be present in measurable amounts.
ISSN:0951-208X
1573-6784
DOI:10.1007/BF01874999