Poly(beta-hydroxyalkanoate) biosynthesis and accumulation by different Rhizobium species

Synthesis and accumulation of poly(β‐hydroxy‐alkanoate) in Rhizobium leguminosarum, R. leguminosarum biovar. trifolii, Rhizobium‘galega’, Rhizobium‘hedysarum’ and R. meliloti were studied. Poly(β‐hydroxybutyrate) is accumulated up to 55% of the cell dry weight. At 30–50% air saturation R. meliloti a...

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Bibliographic Details
Published in:FEMS microbiology letters 1989-08, Vol.60 (3), p.299-304
Main Authors: Tombolini, R, Nuti, M.P
Format: Article
Language:English
Subjects:
3-hydroxybutyric acid
Accumulation
Bacteriology
Biological and medical sciences
Biosynthesis
Fundamental and applied biological sciences. Psychology
Lupinus
Metabolism. Enzymes
Microbiology
nitrogen fixation
Poly(β‐hydroxyalkanoate)
Rhizobium
Rhizobium sp
Vicia faba
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Summary:Synthesis and accumulation of poly(β‐hydroxy‐alkanoate) in Rhizobium leguminosarum, R. leguminosarum biovar. trifolii, Rhizobium‘galega’, Rhizobium‘hedysarum’ and R. meliloti were studied. Poly(β‐hydroxybutyrate) is accumulated up to 55% of the cell dry weight. At 30–50% air saturation R. meliloti accumulates 50% of its biomass (5.5 g·1−1 dry weight) as PHB after 90 h of batch fermentation. At 90% air saturation maximum accumulation (37.5%) of PHB occurs after 70 h cultivation. R. meliloti strain 41 is able to synthesize the copolymer poly(β‐hydroxybutyrate‐co‐β‐hydroxyvalerate) at concentrations up to 58% of the biomass dry weight, containing up to 22 mol%β‐hydroxyvalerate. Different concentrations of both copolymer and hydroxyvalerate were obtained when the microorganism was grown, in batch culture, in the presence of propionate or valerate and with glucose, sucrose or succinate as main carbon source.
ISSN:0378-1097
1574-6968
DOI:10.1111/j.1574-6968.1989.tb03490.x