In vivo imaging in an ABSL-3 regional biocontainment laboratory

Abstract The Regional Biocontainment Laboratory (RBL) at the University of Pittsburgh is a state of the art ABSL 3 facility that supports research on highly pathogenic viruses and bacteria. Recent advances in radiologic imaging provide several noninvasive, in vivo imaging modalities that can be used...

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Bibliographic Details
Published in:Pathogens and disease 2014-07, Vol.71 (2), p.207-212
Main Authors: Scanga, Charles A., Lopresti, Brian J., Tomko, Jaime, Frye, Lonnie J., Coleman, Teresa M., Fillmore, Daniel, Carney, Jonathan P., Lin, Philana L., Flynn, JoAnne L., Gardner, Christina L., Sun, Chengqun, Klimstra, William B., Ryman, Kate D., Reed, Douglas S., Fisher, Daniel J., Cole, Kelly S.
Format: Article
Language:English
Subjects:
ABSL‐3
Animals
Bacterial Infections - diagnosis
Bacterial Infections - pathology
Bioluminescence
Containment of Biohazards
Disease Models, Animal
imaging
in vivo
IVIS
Laboratories
Optical Imaging - methods
Pennsylvania
PET/CT
Radiography
Technology, Radiologic - methods
Virus Diseases - diagnosis
Virus Diseases - pathology
X‐ray
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Summary:Abstract The Regional Biocontainment Laboratory (RBL) at the University of Pittsburgh is a state of the art ABSL 3 facility that supports research on highly pathogenic viruses and bacteria. Recent advances in radiologic imaging provide several noninvasive, in vivo imaging modalities that can be used to longitudinally monitor animals following experimental infection or vaccination. The University of Pittsburgh RBL provides digital radiography, bioluminescence imaging, and PET/CT. Operating these platforms in an ABSL 3 poses unique challenges. This review will discuss the development and refinement of these imaging platforms in high containment, emphasizing specific challenges and how they were overcome. In vivo imaging of highly pathogenic viruses and bacteria allow for real time evaluation of disease progression and treatment without the need for serial sacrifice, an advancement in the development of novel vaccines and therapeutics modalities. Graphical Abstract Figure. In vivo imaging of highly pathogenic viruses and bacteria allow for real time evaluation of disease progression and treatment without the need for serial sacrifice, an advancement in the development of novel vaccines and therapeutics modalities.
ISSN:2049-632X
2049-632X
DOI:10.1111/2049-632X.12186