Enhanced immune responses in pigs by DNA vaccine coexpressing GP3 and GP5 of European type porcine reproductive and respiratory syndrome virus

•The DNA vaccine pVAX1-EU-ORF3-ORF5 were constructed based on the European LV strain.•GP3 could enhance humoral- and cell-mediated immune responses to GP5.•These two proteins delivered by a vaccine can synergistically induce immunity against PRRSV.•Quil A may be useful as an adjuvant for improving t...

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Bibliographic Details
Published in:Journal of virological methods 2014-09, Vol.206, p.27-37
Main Authors: Ren, Jingqiang, Lu, Huijun, Wen, Shubo, Sun, Wenchao, Yan, Fulong, Chen, Xing, Jing, Jie, Liu, Hao, Liu, Cunxia, Xue, Fei, Xiao, Pengpeng, Xin, Shu, Jin, Ningyi
Format: Article
Language:English
Subjects:
Animal Structures - virology
Animals
Antibodies, Neutralizing - blood
Antibodies, Viral - blood
China
Cytokines - blood
Cytokines - secretion
DNA vaccine
European PRRSV
Glycoproteins - genetics
Glycoproteins - immunology
GP3
GP5
Immune responses
Pigs
Porcine Reproductive and Respiratory Syndrome - immunology
Porcine Reproductive and Respiratory Syndrome - prevention & control
Porcine respiratory and reproductive syndrome virus - genetics
Porcine respiratory and reproductive syndrome virus - immunology
Swine
T-Lymphocytes - immunology
Vaccination - methods
Vaccines, DNA - administration & dosage
Vaccines, DNA - genetics
Vaccines, DNA - immunology
Vaccines, Synthetic - administration & dosage
Vaccines, Synthetic - genetics
Vaccines, Synthetic - immunology
Viral Load
Viral Proteins - genetics
Viral Proteins - immunology
Viral Vaccines - administration & dosage
Viral Vaccines - genetics
Viral Vaccines - immunology
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Summary:•The DNA vaccine pVAX1-EU-ORF3-ORF5 were constructed based on the European LV strain.•GP3 could enhance humoral- and cell-mediated immune responses to GP5.•These two proteins delivered by a vaccine can synergistically induce immunity against PRRSV.•Quil A may be useful as an adjuvant for improving the protective efficiency of DNA vaccination against PRRSV in pigs. The European (EU) type of porcine reproductive and respiratory syndrome virus (PRRSV) has recently emerged in China. In this study, three recombinant DNA vaccines, pVAX1-EU-ORF3-ORF5 (coexpressing EU type PRRSV GP3 and GP5), pVAX1-EU-ORF3 and pVAX1-EU-ORF5, were constructed and evaluated for their abilities to induce humoral and cellular responses as well as to protect piglets against homologous virus challenge. All piglets were given booster vaccinations at 21 days after the initial inoculation and then challenged 14 days later. Pigs inoculated with pVAX1-EU-ORF3-ORF5 developed significantly higher (P
ISSN:0166-0934
1879-0984
DOI:10.1016/j.jviromet.2014.05.021