Horseradish peroxidase-catalyzed formation of hydrogels from chitosan and poly(vinyl alcohol) derivatives both possessing phenolic hydroxyl groups

• Published in: Carbohydrate polymers 2014-10, Vol.111, p.404-409
• Main Authors: Sakai, Shinji, Khanmohammadi, Mehdi, Khoshfetrat, Ali Baradar, Taya, Masahito
• Format: Article
• Language: English
• Subjects: Anti-Bacterial Agents - chemistry; Anti-Bacterial Agents - metabolism; Anti-Bacterial Agents - pharmacology; Antibacterial activity; Applied sciences; Biocompatible Materials - chemistry; Biocompatible Materials - metabolism; Biocompatible Materials - pharmacology; Chitosan; Chitosan - chemistry; Chitosan - metabolism; Chitosan - pharmacology; Enzymatic cross-linking; Escherichia coli - drug effects; Escherichia coli Infections - prevention & control; Exact sciences and technology; Horseradish peroxidase; Horseradish Peroxidase - metabolism; Humans; Hydrogel; Hydrogels - chemistry; Hydrogels - metabolism; Hydrogels - pharmacology; Natural polymers; Organic polymers; Phenols - chemistry; Phenols - metabolism; Phenols - pharmacology; Physicochemistry of polymers; Poly(vinyl alcohol); Polyvinyl Alcohol - analogs & derivatives; Polyvinyl Alcohol - metabolism; Polyvinyl Alcohol - pharmacology; Properties and characterization; Solution and gel properties; Starch and polysaccharides
• ISSN: 0144-8617; 1879-1344
• DOI: 10.1016/j.carbpol.2014.05.010

•Hydrogels were obtained from chitosan and PVA derivatives both possessing Ph groups.•Hydrogelation was achieved through horseradish peroxidase-catalyzed reaction.•Gelation time could be controlled from 20s to 2min.•Fibroblastic cells spread on hydrogel of 3% chitosan and 1% PVA derivatives.•E. coli cell growth was inhibited on hydrogel of 3% chitosan and 1% PVA derivatives. Horseradish peroxidase-catalyzed cross-linking was applied to prepare hydrogels from aqueous solutions containing chitosan and poly(vinyl alcohol) derivatives both possessing phenolic hydroxyl groups (denoted as Ph-chitosan and Ph-PVA, respectively). Comparing the hydrogels prepared from the solution of 1.0% (w/v) Ph-chitosan and 3.0% (w/v) Ph-PVA and that of 3.0% (w/v) Ph-chitosan and 1.0% (w/v) Ph-PVA, the gelation time of the former hydrogel was 47s, while was 10s longer than that of the latter one. The breaking point for the former hydrogel under stretching (114% strain) was approximately twice larger than that for the latter one. The swelling ratio of the former hydrogel in saline was about half of the latter one. Fibroblastic cells did not adhere on the former hydrogel but adhered and spread on the latter one. The growth of Escherichia coli cells was fully suppressed on the latter hydrogel during 48h cultivation.