A rationally designed form of the TLR5 agonist, flagellin, supports superior immunogenicity of Influenza B globular head vaccines

• Published in: Vaccine 2014-07, Vol.32 (34), p.4317-4323
• Main Authors: Song, Langzhou, Liu, Ge, Umlauf, Scott, Liu, Xiangyu, Li, Hong, Tian, Haijun, Reiserova, Lucia, Hou, Fuxiang, Bell, Rodney, Tussey, Lynda
• Format: Article
• Language: English
• Subjects: Allergy and Immunology; Amino Acid Sequence; Animals; Applied microbiology; Binding sites; Biological and medical sciences; Cloning; Female; Flagellin - immunology; Flagellin domain 3 insertion; Fundamental and applied biological sciences. Psychology; HA globular head; Hemagglutination Inhibition Tests; Hemagglutinin Glycoproteins, Influenza Virus - immunology; Immunogenicity; Influenza; Influenza A virus; Influenza B vaccine; Influenza B virus - immunology; Influenza Vaccines - immunology; Mice, Inbred BALB C; Microbiology; Molecular Sequence Data; Neutralization Tests; Orthomyxoviridae Infections - prevention & control; Plasmids; Protein Structure, Tertiary; Proteins; Recombinant Fusion Proteins - immunology; Studies; Toll-Like Receptor 5 - agonists; Toll-Like Receptor 5 - immunology; Vaccines; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects); Viruses
• ISSN: 0264-410X; 1873-2518
• DOI: 10.1016/j.vaccine.2014.06.013

Highlights • Designed a novel vaccine format (D3Ins) for fusion of an Influenza B globular head domain and flagellin. • The D3Ins format supports strong TLR5 signaling and consequently robust immunogenicity for Influenza B vaccines. • The D3ins format is generally applicable to both Influenza B lineages. • A D3Ins format Influenza B vaccine protects mice from a lethal challenge. • The D3Ins format allows for prokaryotic production of Influenza B vaccines thereby supporting a time and cost efficient multivalent vaccine product.