Structure of activated sludge flocs

Activated sludge flocs were fixed with formalin, dehydrated with ethanol, cleared and impregnated with liquid paraffin before sectioning with a microtome to a width of 3-6 um. The sections were stained with hematoxilyn eosin, Gram and periodic acid Schiff stains. There was an absence of uniformity i...

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Bibliographic Details
Published in:Biotechnology and bioengineering 1990-01, Vol.35 (1), p.57-65
Main Authors: DA-HONG LI, GANCZARCZYK, J. J
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biotechnology
Environment and pollution
Fundamental and applied biological sciences. Psychology
Industrial applications and implications. Economical aspects
Methods. Procedures. Technologies
Others
Various methods and equipments
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Summary:Activated sludge flocs were fixed with formalin, dehydrated with ethanol, cleared and impregnated with liquid paraffin before sectioning with a microtome to a width of 3-6 um. The sections were stained with hematoxilyn eosin, Gram and periodic acid Schiff stains. There was an absence of uniformity in the structure and in the distribution of micro-organisms in the flocs and there was no characteristic size for the water gaps in the flocs. The distribution of micro-organisms and of extracellular polymers varied randomly. The similarity between the cross-sectional morphology of the floc and the rugged perimeter of the sections might be characterized within a certain size range by the concept of fractals.
ISSN:0006-3592
1097-0290
DOI:10.1002/bit.260350109