Screening multimycotoxins in food-grade gums by stable isotope dilution and liquid chromatography/tandem mass spectrometry

Stable isotope dilution with LC/MSIMS was used to determine the following 11 mycotoxins in food grade gums: aflatoxins B1, B2, G1, and G2; deoxynivalenol; fumonisins B1, B2, and B3; ochratoxin A; T-2 toxin; and zearalenone. Samples were fortified with 11 [13C]-uniformly labeled internal standard ([1...

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Bibliographic Details
Published in:Journal of AOAC International 2014-05, Vol.97 (3), p.889-895
Main Authors: Zhang, Kai, Wong, Jon W, Jia, Zhengwei, Vaclavikova, Marta, Trucksess, Mary W, Begley, Timothy H
Format: Article
Language:English
Subjects:
Carbon Isotopes
Chromatography, Liquid - methods
Contamination
Food contamination
Gums and resins
Health aspects
Identification and classification
Indicator Dilution Techniques
Liquid chromatography
Mass spectrometry
Methods
Mycotoxins
Mycotoxins - analysis
Plant Gums - analysis
Reproducibility of Results
Tandem Mass Spectrometry - methods
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Summary:Stable isotope dilution with LC/MSIMS was used to determine the following 11 mycotoxins in food grade gums: aflatoxins B1, B2, G1, and G2; deoxynivalenol; fumonisins B1, B2, and B3; ochratoxin A; T-2 toxin; and zearalenone. Samples were fortified with 11 [13C]-uniformly labeled internal standard ([13C]-IS) mycotoxins that corresponded to the 11 target mycotoxins and extracted by acetonitrile-water (4 + 1, v/v), followed by LC/MS/MS analysis. Mycotoxins were quantitated with the fortified [13C]-IS in each sample. The average recoveries of aflatoxins B1, B2, G1, and G2 (1, 5, and 25 microg/kg); deoxynivalenol and fumonisins B1, B2, and B3 (25, 100, and 500 microg/kg); and ochratoxin A, T-2 toxin, and zearalenone (10, 50, and 250 microg/kg) ranged from 84 to 117% with RSDs less than 20%. Method-dependent LOQs were from 0.1 (aflatoxin B1) to 25 microg/kg (fumonisin B3). Among 20 market samples, aflatoxin B1 (< LOQ) was detected in a Guar gum and a Tragacanth gum, and zearalenone (6 +/- 0.6 microg/kg) was detected in a Xanthan gum. The detected mycotoxins were further confirmed by comparing their enhanced product ion spectra to those of reference standards. The single laboratory validated stable isotope dilution and LC/MSIMS method provides sufficient selectivity, sensitivity, accuracy, and reproducibility with a simple sample preparation to screen the 11 mycotoxins in gums.
ISSN:1060-3271
1944-7922
DOI:10.5740/jaoacint.13-263