Diversity and potential application of endophytic bacteria in ginger

• Published in: Genetics and molecular research 2014-07, Vol.13 (3), p.4918-4931
• Main Authors: Chen, T, Chen, Z, Ma, G H, Du, B H, Shen, B, Ding, Y Q, Xu, K
• Format: Article
• Language: English
• Subjects: Antibiosis; Antimicrobial Cationic Peptides - biosynthesis; Antimicrobial Cationic Peptides - isolation & purification; Antimicrobial Cationic Peptides - pharmacology; Bacteria - classification; Bacteria - genetics; Bacteria - isolation & purification; Colony Count, Microbial; DNA, Bacterial - genetics; Endophytes - classification; Endophytes - genetics; Endophytes - isolation & purification; Fungi - drug effects; Fungi - growth & development; Fungi - pathogenicity; Microbial Consortia; Phylogeny; Pythium - drug effects; Pythium - growth & development; Pythium - pathogenicity; RNA, Ribosomal, 16S - genetics; Seedlings - microbiology; Zingiber officinale - microbiology
• ISSN: 1676-5680; 1676-5680
• DOI: 10.4238/2014.July.4.6

Here, 248 endophytic bacterial strains were isolated to assess the distribution and population diversity of endophytic bacteria in ginger plants. A total of 10.4 x 10(4) to 20.2 x 10(4) CFU/g fresh weight endophytic bacteria of different growth stages were isolated. Maximum bacterium numbers were obtained at the seedling stage. A total of 107 functional strains were screened, including 17 antibacterial strains and 90 indole acetic acid-producing strains. Based on 16S rDNA sequence restriction fragment length polymorphism and 16S rDNA sequences, these 107 strains were mapped and grouped into 16 genera. Bacillus and Pseudomonas were the dominant genera; however, the bacteria belonged to a tremendous range of genera, with the highest species richness being observed at the seedling stage. Sixteen strains exhibited antimicrobial activity against Pythium myriotylum Drechsler, while 7 strains exhibited antimicrobial activity against Phyllosticta zingiberi Hori. Bacillus was the dominant antibacterial strain. Pseudomonas fluorescens, B. megaterium, and Enterobacter ludwigii produced remarkably high levels of IAA. Only a few endophytic bacterial strains were inhibited in fresh ginger juice. Most of these strains were present during seedling stage, including Roseateles depolymerans, Chryseobacterium taiwanense, E. ludwigii, Agrobacterium larrymoorei, P. fluorescens, and Bacillus amyloliquefaciens. This study indicates that the community of endophytic bacteria in ginger changes with the synthesis of antibacterial substances.