Isolation and characterization of genetic variability in bacteria with β-hemolytic and antifungal activity isolated from the rhizosphere of Medicago truncatula plants

In the present study, we analyzed the frequency of hemolytic and antifungal activities in bacterial isolates from the rhizosphere of Medicago truncatula plants. Of the 2000 bacterial colonies, 96 showed β-hemolytic activities (frequency, 4.8 x 10(-2)). Hemolytic isolates were analyzed for their gene...

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Bibliographic Details
Published in:Genetics and molecular research 2014-07, Vol.13 (3), p.4967-4975
Main Authors: Hernández-Salmerón, J E, Prieto-Barajas, C M, Valencia-Cantero, E, Moreno-Hagelsieb, G, Santoyo, G
Format: Article
Language:English
Subjects:
Antibiosis
Antifungal Agents - metabolism
Antifungal Agents - pharmacology
Bacillus - classification
Bacillus - genetics
Bacillus - metabolism
Bacillus subtilis - classification
Bacillus subtilis - genetics
Bacillus subtilis - metabolism
Bacterial Typing Techniques
Fusarium - drug effects
Fusarium - growth & development
Genetic Variation
Haplotypes
Hemolysis
Medicago truncatula - microbiology
Phylogeny
Random Amplified Polymorphic DNA Technique
Rhizoctonia - drug effects
Rhizoctonia - growth & development
Rhizosphere
RNA, Ribosomal, 16S - genetics
Soil Microbiology
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Summary:In the present study, we analyzed the frequency of hemolytic and antifungal activities in bacterial isolates from the rhizosphere of Medicago truncatula plants. Of the 2000 bacterial colonies, 96 showed β-hemolytic activities (frequency, 4.8 x 10(-2)). Hemolytic isolates were analyzed for their genetic diversity by using random amplification of polymorphic DNA, yielding 88 haplotypes. The similarity coefficient of Nei and Li showed a polymorphic diversity ranging from 0.3 to 1. Additionally, 8 of the hemolytic isolates showed antifungal activity toward plant pathogens, Diaporthe phaseolorum, Colletotrichum acutatum, Rhizoctonia solani, and Fusarium oxysporum. The 16S ribosomal sequencing analysis showed that antagonistic bacterial isolates corresponded to Bacillus subtilis (UM15, UM33, UM42, UM49, UM52, and UM91), Bacillus pumilus (UM24), and Bacillus licheniformis (UM88). The present results revealed a higher genetic diversity among hemolytic isolates compared to that of isolates with antifungal action.
ISSN:1676-5680
1676-5680
DOI:10.4238/2014.July.4.11