Ultrasensitive electrochemical biosensor based on graphite oxide, Prussian blue, and PTC-NH2 for the detection of α2,6-sialylated glycans in human serum

• Published in: Biosensors & bioelectronics 2014-12, Vol.62, p.79-83
• Main Authors: Gao, Liuliu, He, Junlin, Xu, Wailan, Zhang, Jing, Hui, Junmin, Guo, Yanlei, Li, Wenjuan, Yu, Chao
• Format: Article
• Language: English
• Subjects: Anhydrides - chemistry; Biological and medical sciences; Biosensing Techniques - instrumentation; Biosensors; Biotechnology; Electrochemical Techniques - instrumentation; Electrodes; Ferrocyanides - chemistry; Fundamental and applied biological sciences. Psychology; Gold nanoparticles; Graphite - chemistry; Graphite oxide; Humans; Limit of Detection; Methods. Procedures. Technologies; N-Acetylneuraminic Acid - analysis; N-Acetylneuraminic Acid - blood; Oxides - chemistry; Perylene - analogs & derivatives; Perylene - chemistry; Polysaccharides - blood; Polysaccharides - chemistry; Prussian blue; PTC-NH2; Various methods and equipments; α2,6-Sialylated glycans
• ISSN: 0956-5663; 1873-4235
• DOI: 10.1016/j.bios.2014.06.031

α2,6-Sialylated glycans are crucial molecular targets for cancer diagnosis and clinical research. In this work, a novel ultrasensitive electrochemical biosensor was fabricated based on a graphite oxide (GO), Prussian blue (PB), and PTC-NH2 (an ammonolysis product of 3,4,9,10-perylenetetracarboxylic dianhydride) nanocomposite for the selective detection of α2,6-sialylated glycans. To increase the sensitivity of the electrochemical biosensor, gold nanoparticles (GNPs) were immobilized on a GO–PB–PTC-NH2 modified glassy carbon electrode (GCE). Sambucus nigra agglutinins (SNAs), which specifically bind with α2,6-sialylated glycans, were covalently immobilized on GNPs for the sensitive detection of α2,6-sialylated glycans in serum. This proposed method can be applied to human serum, and it worked well over a broad linear range (0.1pgmL−1–500ngmL−1) with detection limits of 0.03pgmL−1. Moreover, recovery of the spiked samples ranged from 100.2% to 105.0%, suggesting that this excellent electrochemical biosensor can be used for the practical detection of α2,6-sialylated glycans. •This paper describes a novel ultrasensitive biosensor for the detection of α2,6-sialylated glycans in human serum.•GO–PB–PTC-NH2 modified GCE displays excellent direct electrochemistry.•The biosensor possesses wide linear range, low detection limit, good specificity and high sensitivity.