Cimetidine attenuates vinorelbine-induced phlebitis in mice by militating E-selectin expression

• Published in: Cancer chemotherapy and pharmacology 2014-08, Vol.74 (2), p.239-247
• Main Authors: Wang, Zhuo, Ma, Lijuan, Wang, Xuebin, Cai, Heping, Huang, Jin, Liu, Jiyong, Hu, Jinhong, Su, Dingfeng
• Format: Article
• Language: English
• Subjects: Animals; Antineoplastic Agents, Phytogenic - toxicity; Blotting, Western; Cancer Research; Cell Proliferation - drug effects; Cells, Cultured; Cimetidine - therapeutic use; E-Selectin - physiology; Endothelium, Vascular - cytology; Endothelium, Vascular - drug effects; Endothelium, Vascular - metabolism; Flow Cytometry; Histamine H2 Antagonists - therapeutic use; Humans; Infusions, Intravenous; Medicine; Medicine & Public Health; Mice; Mice, Inbred C57BL; Mice, Knockout; Oncology; Original Article; Pharmacology/Toxicology; Phlebitis - chemically induced; Phlebitis - drug therapy; Phlebitis - metabolism; Rabbits; Real-Time Polymerase Chain Reaction; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger - genetics; Vinblastine - analogs & derivatives; Vinblastine - toxicity
• ISSN: 0344-5704; 1432-0843
• DOI: 10.1007/s00280-014-2487-8

Purpose We investigated E -selectin expression in mice and rabbits with vinorelbine-induced phlebitis and the effect of cimetidine. To find the relationship between E -selectin expression and vinorelbine-induced phlebitis. Methods Mouse and rabbit model of vinorelbine-induced phlebitis was established by intravenous infusion of vinorelbine. Pathological observation, molecular-biological determination of E -selectin and protein function of it was evaluated. Results Grossly, we observed swelling, edema and cord-like vessel changes in mice receiving vinorelbine but only mild edema in mice pretreated with cimetidine. Pathological scoring yielded a total score of 37 for vinorelbine-treated mice and 17 for mice pretreated with cimetidine ( P