CD45RA-Foxp3(low) non-regulatory T cells in the CCR7-CD45RA-CD27+CD28+ effector memory subset are increased in synovial fluid from patients with rheumatoid arthritis

Increased numbers of regulatory T (Treg) cells are found in synovial fluid from patients with rheumatoid arthritis (RASF) compared with peripheral blood. However, Treg cells in RASF have been shown to have a decreased capacity to suppress T cells. Here we phenotypically classified CD4+ T cells in RA...

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Published in:Cellular immunology 2014-07, Vol.290 (1), p.96-101
Main Authors: Matsuki, Fumichika, Saegusa, Jun, Nishimura, Keisuke, Miura, Yasushi, Kurosaka, Masahiro, Kumagai, Shunichi, Morinobu, Akio
Format: Article
Language:English
Subjects:
Adult
Aged
Arthritis, Rheumatoid - immunology
CD28 Antigens - biosynthesis
CD28 Antigens - immunology
CD8-Positive T-Lymphocytes - classification
Female
Forkhead Transcription Factors - immunology
Humans
Immunologic Memory
Inflammation - immunology
Leukocyte Common Antigens - biosynthesis
Leukocyte Common Antigens - immunology
Male
Middle Aged
Receptors, CCR7 - biosynthesis
Receptors, CCR7 - immunology
Synovial Fluid - cytology
Synovial Fluid - immunology
T-Lymphocytes, Helper-Inducer - classification
T-Lymphocytes, Regulatory - classification
Tumor Necrosis Factor Receptor Superfamily, Member 7 - biosynthesis
Tumor Necrosis Factor Receptor Superfamily, Member 7 - immunology
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Summary:Increased numbers of regulatory T (Treg) cells are found in synovial fluid from patients with rheumatoid arthritis (RASF) compared with peripheral blood. However, Treg cells in RASF have been shown to have a decreased capacity to suppress T cells. Here we phenotypically classified CD4+ T cells in RASF into six subsets based on the expression of CD45RA, CCR7, CD27 and CD28, and demonstrated that the CCR7-CD45RA-CD27+CD28+ TEM subset was significantly increased in synovial fluid compared with peripheral blood. In addition, the proportion of Foxp3+ Treg cells in the CCR7-CD45RA-CD27+CD28+ TEM subset was significantly increased in RASF. Furthermore, most of the Foxp3+ Treg cells in RASF were non-suppressive CD45RA-Foxp3(low) non-Treg cells, and the frequency of the non-Treg cells in the CCR7-CD45RA-CD27+CD28+ TEM subset was significantly increased in RASF. Our findings suggest that the pro-inflammatory environment in RA joints may induce the increase of CD45RA-Foxp3(low) non-Treg cells in synovial fluid.
ISSN:1090-2163
DOI:10.1016/j.cellimm.2014.05.011