Topical Formulations Containing Finasteride. Part II: Determination of Finasteride Penetration into Hair Follicles using the Differential Stripping Technique

The differential stripping technique consists of a tape-stripping phase followed by a cyanoacrylate biopsy. This technique not only allows the quantification of drug retained in the stratum corneum (SC) and in the hair follicles but also differentiates transepidermal from transfollicular penetration...

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Bibliographic Details
Published in:Journal of pharmaceutical sciences 2014-08, Vol.103 (8), p.2323-2329
Main Authors: Tampucci, Silvia, Burgalassi, Susi, Chetoni, Patrizia, Lenzi, Carla, Pirone, Andrea, Mailland, Federico, Caserini, Maurizio, Monti, Daniela
Format: Article
Language:English
Subjects:
5-alpha Reductase Inhibitors - administration & dosage
5-alpha Reductase Inhibitors - pharmacokinetics
Administration, Cutaneous
Animals
differential stripping technique
distribution
finasteride
Finasteride - administration & dosage
Finasteride - pharmacokinetics
formulation
Hair Follicle - metabolism
Hair Follicle - ultrastructure
hair follicles
hydroxypropyl chitosan
in vitro models
Male
passive diffusion/transport
Rats
Rats, Hairless
skin
Skin - metabolism
Skin - ultrastructure
Skin Absorption
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Summary:The differential stripping technique consists of a tape-stripping phase followed by a cyanoacrylate biopsy. This technique not only allows the quantification of drug retained in the stratum corneum (SC) and in the hair follicles but also differentiates transepidermal from transfollicular penetration. Our study aimed at both validating the differential stripping procedure on hairless rat skin and assessing the role of the hair follicle in the cutaneous penetration of finasteride (FNS) after application of two experimental formulations for 6 or 24 h: P-08–016, a hydroxypropyl chitosan (HPCH)-based formulation and P-10–008, an anhydrous formulation devoid of HPCH. Microscopic and histological evaluation showed that after 15 tape strips both the SC and the viable epidermis were completely removed. A subsequent cyanoacrylate skin surface biopsy led to the removal of the infundibula content. The largest amounts of FNS were found in the epidermis and in the appendages after application of P-08–016, regardless of the time from application. In contrast, smaller and statistically significant amounts of FNS were recovered with P-10–008 6h after application, compared with that at 24 h. In conclusion, the differential stripping technique allowed determination of the amount of FNS localized in different skin districts, focusing particularly on the follicular contribution. © 2014 Wiley Periodicals, Inc. and the American Pharmacists Association J Pharm Sci 103:2323–2329, 2014
ISSN:0022-3549
1520-6017
DOI:10.1002/jps.24045