Increased expression of sialyl-dimeric Le super(x) antigen in liver metastases of human colorectal carcinoma

We collected a total of 78 tissue specimens, including primary colorectal carcinoma, normal colonic mucosa, and liver metastases of colon carcinoma, to examine whether the extracts of these tissues inhibited the binding of a monoclonal antibody FH6, specific for sialyl-dimeric Le super(x) antigen. E...

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Bibliographic Details
Published in:Cancer research (Chicago, Ill.) Ill.), 1989-01, Vol.49 (24), p.6883-6888
Main Authors: Hoff, S D, Matsushita, Y, Ota, D M, Cleary, K R, Yamori, T, Hakomori, S, Irimura, T
Format: Article
Language:English
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Summary:We collected a total of 78 tissue specimens, including primary colorectal carcinoma, normal colonic mucosa, and liver metastases of colon carcinoma, to examine whether the extracts of these tissues inhibited the binding of a monoclonal antibody FH6, specific for sialyl-dimeric Le super(x) antigen. Electrophoretic separation of tumor tissue extracts on 3% polyacrylamide gels followed by direct staining with monoclonal antibody FH6 revealed that very high molecular weight glycoproteins, presumably mucins, contained sialyl-dimeric Le super(x) antigen.
ISSN:0008-5472