H-2K super(k) and Vesicular Stomatitis Virus G Proteins are not Extensively Associated in Reconstituted Membranes Recognized by T Cells

It is shown that liposomes containing (i) a fluorescein-labeled murine histocompatibility antigen (FITC-H-2K super(k)) and the G protein of vesicular stomatitis virus or (ii) H-2K super(k) and fluorescein-labeled viral protein (FITC-G) can elicit H-2-restricted syngeneic antiviral cytotoxic T cells...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1982-01, Vol.79 (5), p.1506-1510
Main Authors: Cartwright, G S, Smith, L M, Heinzelmann, E W, Ruebush, MJ, Parce, J W, McConnell, H M
Format: Article
Language:English
Subjects:
histocompatibility antigen H-2
liposomes
lymphocytes T
mice
proteins
reconstitution
vesicular stomatitis virus
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Summary:It is shown that liposomes containing (i) a fluorescein-labeled murine histocompatibility antigen (FITC-H-2K super(k)) and the G protein of vesicular stomatitis virus or (ii) H-2K super(k) and fluorescein-labeled viral protein (FITC-G) can elicit H-2-restricted syngeneic antiviral cytotoxic T cells as assayed by super(51)Cr release from appropriate virus-infected target cells. Fluorescence recovery after photobleaching was used to measure the diffusion coefficients of these reconstituted proteins in four different samples: (i) FITC-H-2K super(k); (ii) FITC-H-2K super(k) and G; (iii) FITC-G; and (iv) FITC-G and H-2K super(k). The same rate of lateral diffusion (D = 1 x 10 super(-8) cm super(2)/sec at 37 degree C in 25% cholesterol/75% dimyristoylphosphatidylcholine) was obtained in every case. Both proteins, fluorescent as well as nonfluorescent, could be patched by using specific antibodies. When G was patched with antibody, FITC-H-2K super(k) did not copatch. When H-2K super(k) was patched with antibody, FITC-G did not copatch. These diffusion and patching measurements rule out the possibility that these proteins have either extensive oligomeric associations or strong specific pairwise associations.
ISSN:0027-8424