Cloning and structural characterization of an estrogen-dependent apolipoprotein gene

ApoVLDLII is a major polypeptide component of avian very low density lipoprotein. Its production in the liver of the maturing female chick is developmentally synchronized with vitellogenesis. The apoVLDLII gene is normally dormant in males but can be activated by the administration of estrogen. In t...

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Bibliographic Details
Published in:The Journal of biological chemistry 1981-09, Vol.256 (18), p.9662-9667
Main Authors: Wiskocil, R, Goldman, P, Deeley, R G
Format: Article
Language:English
Subjects:
Animals
Apolipoproteins - genetics
Chickens
Cloning, Molecular
DNA Restriction Enzymes
DNA, Recombinant - metabolism
Escherichia coli - genetics
Estrogens - pharmacology
Female
Genes - drug effects
Lipoproteins, VLDL - genetics
Male
Microscopy, Electron
Nucleic Acid Hybridization
Plasmids
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Summary:ApoVLDLII is a major polypeptide component of avian very low density lipoprotein. Its production in the liver of the maturing female chick is developmentally synchronized with vitellogenesis. The apoVLDLII gene is normally dormant in males but can be activated by the administration of estrogen. In these studies, we describe the isolation of three recombinant bacteriophages that contain apoVLDLII genes. The genes appear to be identical from preliminary restriction analyses, although heterogeneity in flanking sequences is evident. The structure of the gene has been characterized and its organization correlated with the structure of the apoVLDLII polypeptide.
ISSN:0021-9258
1083-351X
DOI:10.1016/S0021-9258(19)68813-7