Microbial Transglutaminase Treatment in Pasta-Production Does Not Affect the Immunoreactivity of Gliadin with Celiac Disease Patients’ Sera

The effect of microbial transglutaminase (MTG)-treatment of pasta-dough on the immunoreactivity with celiac disease patient’s sera has been investigated. Modification by MTG has been proven by determination of the MTG reaction product ε-(γ-glutamyl)­lysine (3.63 μmol/g protein), which was not detect...

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Bibliographic Details
Published in:Journal of agricultural and food chemistry 2014-07, Vol.62 (30), p.7604-7611
Main Authors: Ruh, Tobias, Ohsam, Jürgen, Pasternack, Ralf, Yokoyama, Keiichi, Kumazawa, Yoshiyuki, Hils, Martin
Format: Article
Language:English
Subjects:
Adolescent
Adult
Aged
Aged, 80 and over
antibodies
Antibody Formation - drug effects
celiac disease
Celiac Disease - blood
Celiac Disease - immunology
Child
Child, Preschool
deamidation
Dipeptides - metabolism
Enzyme-Linked Immunosorbent Assay
Food Handling - methods
gliadin
Gliadin - analysis
Gliadin - immunology
Humans
Immunoblotting
Infant
lysine
Middle Aged
pasta
patients
protein-glutamine gamma-glutamyltransferase
Streptomyces - enzymology
Transglutaminases - chemistry
Triticum - chemistry
Western blotting
Young Adult
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Summary:The effect of microbial transglutaminase (MTG)-treatment of pasta-dough on the immunoreactivity with celiac disease patient’s sera has been investigated. Modification by MTG has been proven by determination of the MTG reaction product ε-(γ-glutamyl)­lysine (3.63 μmol/g protein), which was not detectable in non-MTG-treated pasta. Antigenicity has been analyzed by immunoblotting and ELISA using gliadin-extracts from pasta and MTG-treated pasta. Immunoblotting showed that the antibody-population (antigliadin antibodies and antideamidated gliadin antibodies) of the sera is specific for every individual patient. Immunoblotting and ELISA showed that there is no difference in immunoreactivity of gliadin extracted from pasta and MTG-pasta. Recognition pattern and intensity in Western blot as well as antibody titer has also been identical even for sera with a high antideamidated gliadin antibody titer. These results indicate no difference between pasta-gliadin and MTG-pasta-gliadin and especially no increased deamidation in pasta-gliadin by MTG-treatment.
ISSN:0021-8561
1520-5118
DOI:10.1021/jf501275c