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Fetal jaw movement affects Ihh signaling in mandibular condylar cartilage development: The possible role of Ihh as mechanotransduction mediator

Abstract Objective Jaw movement is an important mechanical factor for prenatal development of the condylar cartilage of mandible. Fetal jaw movement restriction has been shown to cause deformity of the mandibular condyle. We hypothesized that this treatment affects the expression of mechanosensitive...

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Published in:Archives of oral biology 2014-10, Vol.59 (10), p.1108-1118
Main Authors: Jahan, Esrat, Matsumoto, Akihiro, Rafiq, Ashiq Mahmood, Hashimoto, Ryuju, Inoue, Takayuki, Udagawa, Jun, Sekine, Joji, Otani, Hiroki
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cited_by cdi_FETCH-LOGICAL-c498t-679b0762d4cb99f358dff9eab770cbdc84a43e18fc1877f792a80774ba638c8a3
cites cdi_FETCH-LOGICAL-c498t-679b0762d4cb99f358dff9eab770cbdc84a43e18fc1877f792a80774ba638c8a3
container_end_page 1118
container_issue 10
container_start_page 1108
container_title Archives of oral biology
container_volume 59
creator Jahan, Esrat
Matsumoto, Akihiro
Rafiq, Ashiq Mahmood
Hashimoto, Ryuju
Inoue, Takayuki
Udagawa, Jun
Sekine, Joji
Otani, Hiroki
description Abstract Objective Jaw movement is an important mechanical factor for prenatal development of the condylar cartilage of mandible. Fetal jaw movement restriction has been shown to cause deformity of the mandibular condyle. We hypothesized that this treatment affects the expression of mechanosensitive molecules, namely Indian hedgehog ( Ihh ) and Parathyroid hormone related protein ( PTHrP ) in the condyle. Experimental methods We restrained jaw movement by suturing the jaw of E15.5 mouse embryos and allowed them to develop until E18.5 using exo utero system, and analyzed them by immunohistochemistry and in situ hybridization methods. Results Morphological, histomorphometric and immunohistochemical study showed that the mandibular condylar cartilage was reduced and deformed, the volume and total cell numbers in the condylar cartilage were also reduced, and number and/or distribution of 5-bromo-2′-deoxyuridine-positive cells, Ihh-positive cells in the mesenchymal and pre-hypertrophic zones were significantly and correspondingly decreased in the sutured group. Using in situ hybridization , reduced expression of Ihh , PTHrP and their related receptors were observed in condylar cartilage of the sutured embryos. Conclusions Our results revealed that the altered mechanical stress induced by prenatal jaw movement restriction decreased proliferating cells, the amount of cartilage, and altered expression of the Ihh and PTHrP , suggesting that Ihh act as mechanotransduction mediators in the development of mandibular condylar cartilage.
doi_str_mv 10.1016/j.archoralbio.2014.06.009
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Fetal jaw movement restriction has been shown to cause deformity of the mandibular condyle. We hypothesized that this treatment affects the expression of mechanosensitive molecules, namely Indian hedgehog ( Ihh ) and Parathyroid hormone related protein ( PTHrP ) in the condyle. Experimental methods We restrained jaw movement by suturing the jaw of E15.5 mouse embryos and allowed them to develop until E18.5 using exo utero system, and analyzed them by immunohistochemistry and in situ hybridization methods. Results Morphological, histomorphometric and immunohistochemical study showed that the mandibular condylar cartilage was reduced and deformed, the volume and total cell numbers in the condylar cartilage were also reduced, and number and/or distribution of 5-bromo-2′-deoxyuridine-positive cells, Ihh-positive cells in the mesenchymal and pre-hypertrophic zones were significantly and correspondingly decreased in the sutured group. Using in situ hybridization , reduced expression of Ihh , PTHrP and their related receptors were observed in condylar cartilage of the sutured embryos. Conclusions Our results revealed that the altered mechanical stress induced by prenatal jaw movement restriction decreased proliferating cells, the amount of cartilage, and altered expression of the Ihh and PTHrP , suggesting that Ihh act as mechanotransduction mediators in the development of mandibular condylar cartilage.</description><identifier>ISSN: 0003-9969</identifier><identifier>EISSN: 1879-1506</identifier><identifier>DOI: 10.1016/j.archoralbio.2014.06.009</identifier><identifier>PMID: 25033382</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Advanced Basic Science ; Animals ; Cartilage, Articular - embryology ; Condylar cartilage ; Dentistry ; Female ; Fetal Development ; Fetal jaw movement ; Hedgehog Proteins - metabolism ; Hedgehog Proteins - physiology ; Ihh ; Immunohistochemistry ; In Situ Hybridization ; Mandibular Condyle - embryology ; Mechanical stress ; Mechanotransduction, Cellular - physiology ; Mice ; Parathyroid Hormone-Related Protein - metabolism ; Parathyroid Hormone-Related Protein - physiology ; Pregnancy ; PTHrP ; Signal Transduction - physiology ; Staining and Labeling ; Stress, Mechanical ; Suture Techniques</subject><ispartof>Archives of oral biology, 2014-10, Vol.59 (10), p.1108-1118</ispartof><rights>Elsevier Ltd</rights><rights>2014 Elsevier Ltd</rights><rights>Copyright © 2014 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c498t-679b0762d4cb99f358dff9eab770cbdc84a43e18fc1877f792a80774ba638c8a3</citedby><cites>FETCH-LOGICAL-c498t-679b0762d4cb99f358dff9eab770cbdc84a43e18fc1877f792a80774ba638c8a3</cites><orcidid>0000-0003-2825-6510</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25033382$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Jahan, Esrat</creatorcontrib><creatorcontrib>Matsumoto, Akihiro</creatorcontrib><creatorcontrib>Rafiq, Ashiq Mahmood</creatorcontrib><creatorcontrib>Hashimoto, Ryuju</creatorcontrib><creatorcontrib>Inoue, Takayuki</creatorcontrib><creatorcontrib>Udagawa, Jun</creatorcontrib><creatorcontrib>Sekine, Joji</creatorcontrib><creatorcontrib>Otani, Hiroki</creatorcontrib><title>Fetal jaw movement affects Ihh signaling in mandibular condylar cartilage development: The possible role of Ihh as mechanotransduction mediator</title><title>Archives of oral biology</title><addtitle>Arch Oral Biol</addtitle><description>Abstract Objective Jaw movement is an important mechanical factor for prenatal development of the condylar cartilage of mandible. Fetal jaw movement restriction has been shown to cause deformity of the mandibular condyle. We hypothesized that this treatment affects the expression of mechanosensitive molecules, namely Indian hedgehog ( Ihh ) and Parathyroid hormone related protein ( PTHrP ) in the condyle. Experimental methods We restrained jaw movement by suturing the jaw of E15.5 mouse embryos and allowed them to develop until E18.5 using exo utero system, and analyzed them by immunohistochemistry and in situ hybridization methods. Results Morphological, histomorphometric and immunohistochemical study showed that the mandibular condylar cartilage was reduced and deformed, the volume and total cell numbers in the condylar cartilage were also reduced, and number and/or distribution of 5-bromo-2′-deoxyuridine-positive cells, Ihh-positive cells in the mesenchymal and pre-hypertrophic zones were significantly and correspondingly decreased in the sutured group. Using in situ hybridization , reduced expression of Ihh , PTHrP and their related receptors were observed in condylar cartilage of the sutured embryos. 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Matsumoto, Akihiro ; Rafiq, Ashiq Mahmood ; Hashimoto, Ryuju ; Inoue, Takayuki ; Udagawa, Jun ; Sekine, Joji ; Otani, Hiroki</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c498t-679b0762d4cb99f358dff9eab770cbdc84a43e18fc1877f792a80774ba638c8a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Advanced Basic Science</topic><topic>Animals</topic><topic>Cartilage, Articular - embryology</topic><topic>Condylar cartilage</topic><topic>Dentistry</topic><topic>Female</topic><topic>Fetal Development</topic><topic>Fetal jaw movement</topic><topic>Hedgehog Proteins - metabolism</topic><topic>Hedgehog Proteins - physiology</topic><topic>Ihh</topic><topic>Immunohistochemistry</topic><topic>In Situ Hybridization</topic><topic>Mandibular Condyle - embryology</topic><topic>Mechanical stress</topic><topic>Mechanotransduction, Cellular - physiology</topic><topic>Mice</topic><topic>Parathyroid Hormone-Related Protein - metabolism</topic><topic>Parathyroid Hormone-Related Protein - physiology</topic><topic>Pregnancy</topic><topic>PTHrP</topic><topic>Signal Transduction - physiology</topic><topic>Staining and Labeling</topic><topic>Stress, Mechanical</topic><topic>Suture Techniques</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Jahan, Esrat</creatorcontrib><creatorcontrib>Matsumoto, Akihiro</creatorcontrib><creatorcontrib>Rafiq, Ashiq Mahmood</creatorcontrib><creatorcontrib>Hashimoto, Ryuju</creatorcontrib><creatorcontrib>Inoue, Takayuki</creatorcontrib><creatorcontrib>Udagawa, Jun</creatorcontrib><creatorcontrib>Sekine, Joji</creatorcontrib><creatorcontrib>Otani, Hiroki</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Archives of oral biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jahan, Esrat</au><au>Matsumoto, Akihiro</au><au>Rafiq, Ashiq Mahmood</au><au>Hashimoto, Ryuju</au><au>Inoue, Takayuki</au><au>Udagawa, Jun</au><au>Sekine, Joji</au><au>Otani, Hiroki</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Fetal jaw movement affects Ihh signaling in mandibular condylar cartilage development: The possible role of Ihh as mechanotransduction mediator</atitle><jtitle>Archives of oral biology</jtitle><addtitle>Arch Oral Biol</addtitle><date>2014-10-01</date><risdate>2014</risdate><volume>59</volume><issue>10</issue><spage>1108</spage><epage>1118</epage><pages>1108-1118</pages><issn>0003-9969</issn><eissn>1879-1506</eissn><abstract>Abstract Objective Jaw movement is an important mechanical factor for prenatal development of the condylar cartilage of mandible. Fetal jaw movement restriction has been shown to cause deformity of the mandibular condyle. We hypothesized that this treatment affects the expression of mechanosensitive molecules, namely Indian hedgehog ( Ihh ) and Parathyroid hormone related protein ( PTHrP ) in the condyle. Experimental methods We restrained jaw movement by suturing the jaw of E15.5 mouse embryos and allowed them to develop until E18.5 using exo utero system, and analyzed them by immunohistochemistry and in situ hybridization methods. Results Morphological, histomorphometric and immunohistochemical study showed that the mandibular condylar cartilage was reduced and deformed, the volume and total cell numbers in the condylar cartilage were also reduced, and number and/or distribution of 5-bromo-2′-deoxyuridine-positive cells, Ihh-positive cells in the mesenchymal and pre-hypertrophic zones were significantly and correspondingly decreased in the sutured group. Using in situ hybridization , reduced expression of Ihh , PTHrP and their related receptors were observed in condylar cartilage of the sutured embryos. Conclusions Our results revealed that the altered mechanical stress induced by prenatal jaw movement restriction decreased proliferating cells, the amount of cartilage, and altered expression of the Ihh and PTHrP , suggesting that Ihh act as mechanotransduction mediators in the development of mandibular condylar cartilage.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>25033382</pmid><doi>10.1016/j.archoralbio.2014.06.009</doi><tpages>11</tpages><orcidid>https://orcid.org/0000-0003-2825-6510</orcidid></addata></record>
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subjects Advanced Basic Science
Animals
Cartilage, Articular - embryology
Condylar cartilage
Dentistry
Female
Fetal Development
Fetal jaw movement
Hedgehog Proteins - metabolism
Hedgehog Proteins - physiology
Ihh
Immunohistochemistry
In Situ Hybridization
Mandibular Condyle - embryology
Mechanical stress
Mechanotransduction, Cellular - physiology
Mice
Parathyroid Hormone-Related Protein - metabolism
Parathyroid Hormone-Related Protein - physiology
Pregnancy
PTHrP
Signal Transduction - physiology
Staining and Labeling
Stress, Mechanical
Suture Techniques
title Fetal jaw movement affects Ihh signaling in mandibular condylar cartilage development: The possible role of Ihh as mechanotransduction mediator
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