NADPH oxidase hyperactivity induces plantaris atrophy in heart failure rats

• Published in: International journal of cardiology 2014-08, Vol.175 (3), p.499-507
• Main Authors: Bechara, Luiz R.G, Moreira, Jose B.N, Jannig, Paulo R, Voltarelli, Vanessa A, Dourado, Paulo M, Vasconcelos, Andrea R, Scavone, Cristoforo, Ramires, Paulo R, Brum, Patricia C
• Format: Article
• Language: English
• Subjects: Animals; Biological and medical sciences; Cardiology. Vascular system; Cardiovascular; Enzyme Activation - physiology; Heart; Heart Failure - enzymology; Heart Failure - pathology; Heart failure, cardiogenic pulmonary edema, cardiac enlargement; Male; Medical sciences; Membrane Glycoproteins - metabolism; Muscle, Skeletal - enzymology; Muscle, Skeletal - pathology; Muscular Atrophy - enzymology; Muscular Atrophy - pathology; NADPH Oxidase 2; NADPH Oxidases - metabolism; Rats; Rats, Wistar; Reactive Oxygen Species - metabolism
• ISSN: 0167-5273; 1874-1754
• DOI: 10.1016/j.ijcard.2014.06.046

Abstract Background Skeletal muscle wasting is associated with poor prognosis and increased mortality in heart failure (HF) patients. Glycolytic muscles are more susceptible to catabolic wasting than oxidative ones. This is particularly important in HF since glycolytic muscle wasting is associated with increased levels of reactive oxygen species (ROS). However, the main ROS sources involved in muscle redox imbalance in HF have not been characterized. Therefore, we hypothesized that NADPH oxidases would be hyperactivated in the plantaris muscle of infarcted rats, contributing to oxidative stress and hyperactivation of the ubiquitin-proteasome system (UPS), ultimately leading to atrophy. Methods Rats were submitted to myocardial infarction (MI) or Sham surgery. Four weeks after surgery, MI and Sham groups underwent eight weeks of treatment with apocynin, a NADPH oxidase inhibitor, or placebo. NADPH oxidase activity, oxidative stress markers, NF-κB activity, p38 MAPK phosphorylation, mRNA and sarcolemmal protein levels of NADPH oxidase components, UPS activation and fiber cross-sectional area were assessed in the plantaris muscle. Results The plantaris of MI rats displayed atrophy associated with increased Nox2 mRNA and sarcolemmal protein levels, NADPH oxidase activity, ROS production, lipid hydroperoxides levels, NF-κB activity, p38 MAPK phosphorylation and UPS activation. NADPH oxidase inhibition by apocynin prevented MI-induced skeletal muscle atrophy by reducing ROS production, NF-κB hyperactivation, p38 MAPK phosphorylation and proteasomal hyperactivity. Conclusion Our data provide evidence for NADPH oxidase hyperactivation as an important source of ROS production leading to plantaris atrophy in heart failure rats, suggesting that this enzyme complex plays key role in skeletal muscle wasting in HF.