Purification of High-Molecular-Weight Subfraction from Porcine Skin Inhibiting Proliferation of A431 Human Carcinoma Epidermoid Cells

• Published in: Bulletin of experimental biology and medicine 2014-07, Vol.157 (3), p.371-374
• Main Authors: Belova, O. V., Sergienko, V. I., Arion, V. Ya, Lukanidina, T. A., Moskvina, S. N., Zimina, I. V., Borisenko, G. G., Lutsenko, G. V., Grechikhina, M. V., Kovaleva, E. V., Klyuchnikova, Zh. I.
• Format: Article
• Language: English
• Subjects: Animals; Antineoplastic Agents - isolation & purification; Antineoplastic Agents - pharmacology; Biomedical and Life Sciences; Biomedicine; Cancer; Carcinoma; Cell Biology; Cell Line, Tumor; Cell Proliferation - drug effects; Chromatography, Ion Exchange; Drug Screening Assays, Antitumor; Hostages; Humans; Inhibitory Concentration 50; Internal Medicine; Laboratory Medicine; Molecular Weight; Pathology; Proteins; Skin; Skin - chemistry; Sus scrofa; Tissue Extracts - isolation & purification; Tissue Extracts - pharmacology
• ISSN: 0007-4888; 1573-8221
• DOI: 10.1007/s10517-014-2569-y

Subfraction with a molecular weight >250 kDa isolated from porcine skin and inhibiting the proliferation of A431 human carcinoma epidermoid cells was purifi ed by DEAE 32 anion exchange chromatography with NaCl concentration step-gradient. The effects of the initial subfraction and fractions obtained by separation in DEAE 32 on the proliferation of A431 human carcinoma epidermoid cells were studied in vitro in two tests (MTT and fl uorescent test). The more sensitive fl uorescent test showed the highest inhibitory activity of fraction No. 2 released from the column at 0.15 M NaCl. One major protein component and a series of minor protein components were detected in this fraction by vertical PAAG-SDS electrophoresis.