Simultaneous and rapid determination of caffeine and taurine in energy drinks by MEKC in a short capillary with dual contactless conductivity/photometry detection

A method has been developed for the simultaneous determination of taurine and caffeine using a laboratory‐made instrument enabling separation analysis in a short 10.5 cm capillary. The substances are detected using a contactless conductometry/ultraviolet (UV) photometry detector that enables recordi...

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Bibliographic Details
Published in:Electrophoresis 2014-06, Vol.35 (11), p.1660-1665
Main Authors: Vochyánová, Blanka, Opekar, František, Tůma, Petr
Format: Article
Language:English
Subjects:
Caffeine
Caffeine - analysis
Capillarity
Chromatography, Micellar Electrokinetic Capillary - economics
Chromatography, Micellar Electrokinetic Capillary - instrumentation
Conductometry - economics
Conductometry - instrumentation
Drinks
Dual detection
Electric Conductivity
Energy drinks
Energy Drinks - analysis
Equipment Design
Limit of Detection
Micellar electrokinetic chromatography
Migration
Photometry
Photometry - economics
Photometry - instrumentation
Recording
Separation
Short capillary separation
Taurine
Taurine - analysis
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Summary:A method has been developed for the simultaneous determination of taurine and caffeine using a laboratory‐made instrument enabling separation analysis in a short 10.5 cm capillary. The substances are detected using a contactless conductometry/ultraviolet (UV) photometry detector that enables recording both signals at one place in the capillary. The separation of caffeine and taurine was performed using the MEKC technique in a BGE with the composition 40 mM CHES, 15 mM NaOH, and 50 mM SDS, pH 9.36. Under these conditions, the migration time of caffeine is 43 s and of taurine 60 s; LOD for caffeine is 4 mg/L using photometric detection and LOD for taurine is 24 mg/L using contactless conductometric detection. The standard addition method was used for determination in Red Bull energy drink of caffeine 317 mg/L and taurine 3860 mg/L; the contents in Kamikaze drink were 468 mg/L caffeine and 4110 mg/L taurine. The determined values are in good agreement with the declared contents of these substances. RSD does not exceed 3%.
ISSN:0173-0835
1522-2683
DOI:10.1002/elps.201300480