Prevalence and genotypes of extended spectrum beta-lactamases in Enterobacteriaceae isolated from human stool and chicken meat in Hamburg, Germany

• Published in: International journal of medical microbiology 2014-07, Vol.304 (5), p.678-684
• Main Authors: Belmar Campos, Cristina, Fenner, Ines, Wiese, Nicole, Lensing, Carmen, Christner, Martin, Rohde, Holger, Aepfelbacher, Martin, Fenner, Thomas, Hentschke, Moritz
• Format: Article
• Language: English
• Subjects: Adolescent; Adult; Aged; Aged, 80 and over; Animals; Anti-Bacterial Agents - pharmacology; beta-Lactamases - genetics; Chickens; Child; Child, Preschool; Drug Resistance, Bacterial; Emergency department; Enterobacteriaceae; Enterobacteriaceae - classification; Enterobacteriaceae - enzymology; Enterobacteriaceae - genetics; Enterobacteriaceae - isolation & purification; ESBL; Escherichia coli; Feces - microbiology; Female; Genotype; Germany; Humans; Infant; Infectious Disease; Male; Meat - microbiology; Medical Education; Middle Aged; Multilocus Sequence Typing; Poultry; Prevalence; Sequence type; Young Adult
• ISSN: 1438-4221; 1618-0607
• DOI: 10.1016/j.ijmm.2014.04.012

Abstract Chicken meat has been proposed to constitute a source for extended spectrum beta-lactamase (ESBL)-carrying Enterobacteriaceae that colonize and infect humans. In this study the prevalence of ESBL-producing Enterobacteriaceae in stool samples from ambulatory patients who presented in the emergency department of the University Medical Centre Hamburg-Eppendorf with gastrointestinal complains and in chicken meat samples from the Hamburg region were analysed and compared with respect to ESBL-genotypes, sequence types and antibiotic resistance profiles. Twenty-nine (4.1%) of 707 stool samples and 72 (60%) of 120 chicken meat samples were positive for ESBL-producing Enterobacteriaceae . The distribution of ESBL genes in the stool vs. chicken meat isolates (given as % of total isolates from stool vs. chicken meat) was as follows: CTX-M-15 (38% vs. 0%), CTX-M-14 (17% vs. 6%), CTX-M-1 (17% vs. 69%), SHV-12 (3% vs. 18%) and TEM-52 (3% each). Comparison of ESBL- and multilocus sequence type revealed no correlation between isolates of human and chicken. Furthermore, ESBL-producing E. coli from stool samples were significantly more resistant to fluoroquinolones, aminoglycosides and/or trimethoprim-sulfamethoxazole than chicken isolates. The differences in ESBL-genotypes, sequence types and antibiotic resistance patterns indicate that in our clinical setting chicken meat is not a major contributor to human colonization with ESBL-carrying Enterobacteriaceae.