PCR array analysis of gene expression profiles in chronic active Epstein–Barr virus infection

To determine the host cellular gene expression profiles in chronic active Epstein–Barr virus infection (CAEBV), peripheral blood samples were obtained from three patients with CAEBV and investigated using a PCR array analysis that focused on T-cell/B-cell activation. We identified six genes with exp...

Full description

Saved in:
Bibliographic Details
Published in:Microbes and infection 2014-07, Vol.16 (7), p.581-586
Main Authors: Murakami, Masanao, Hashida, Yumiko, Imajoh, Masayuki, Maeda, Akihiko, Kamioka, Mikio, Senda, Yasutaka, Sato, Tetsuya, Fujieda, Mikiya, Wakiguchi, Hiroshi, Daibata, Masanori
Format: Article
Language:English
Subjects:
CAEBV
Case-Control Studies
Chronic Disease
Cytokine
Cytokines - analysis
Cytokines - genetics
Cytokines - metabolism
Epstein-Barr virus
Epstein-Barr Virus Infections - blood
Epstein-Barr Virus Infections - genetics
Epstein-Barr Virus Infections - metabolism
Female
Gene Expression Profiling - methods
Gene profile
Humans
Male
PCR array
Polymerase Chain Reaction - methods
Transcriptome - genetics
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:To determine the host cellular gene expression profiles in chronic active Epstein–Barr virus infection (CAEBV), peripheral blood samples were obtained from three patients with CAEBV and investigated using a PCR array analysis that focused on T-cell/B-cell activation. We identified six genes with expression levels that were tenfold higher in CAEBV patients compared with those in healthy controls. These results were verified by quantitative reverse transcription-PCR. We identified four highly upregulated genes, i.e., IL-10, IL-2, IFNGR1, and INHBA. These genes may be involved in inflammatory responses and cell proliferation, and they may contribute to the development and progression of CAEBV.
ISSN:1286-4579
1769-714X
DOI:10.1016/j.micinf.2014.04.004