Expression and identification of a novel gene Spata34 in mouse spermatogenic cells

Leucine-rich repeat (LRR) containing proteins play an essential role in signal transduction, cell adhesion, cell development, DNA repair and RNA processing. Here we cloned a novel gene, Spata34 , encoding a LRR containing protein of 415 aa. Spata34 gene consisted of 9 exons and 8 introns and mapped...

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Bibliographic Details
Published in:Molecular biology reports 2014-03, Vol.41 (3), p.1683-1691
Main Authors: Chen, Tingfang, Jiang, Zhigang, Xu, Wei, Wang, Yuequn, Li, Yongqing, Wan, Yongqi, Yuan, Wuzhou, Mo, Xiaoyang, Wu, Xiushan, Deng, Yun, Fan, Xiongwei, Nie, Dongsong
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animal Anatomy
Animal Biochemistry
Animals
Biomedical and Life Sciences
Cercopithecus aethiops
Cloning
COS Cells
Female
Gene expression
Gene Expression Regulation, Developmental
Germ Cells - growth & development
Germ Cells - metabolism
Histology
Humans
Life Sciences
Male
Membrane Proteins - biosynthesis
Membrane Proteins - genetics
Mice
Molecular biology
Morphology
Repressor Proteins - genetics
Rodents
Sperm
Spermatids - growth & development
Spermatids - metabolism
Spermatogenesis - genetics
Testis - growth & development
Testis - metabolism
Transcription Factor AP-1 - biosynthesis
Transcription Factor AP-1 - metabolism
Tumor Suppressor Protein p53 - biosynthesis
Tumor Suppressor Protein p53 - metabolism
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Summary:Leucine-rich repeat (LRR) containing proteins play an essential role in signal transduction, cell adhesion, cell development, DNA repair and RNA processing. Here we cloned a novel gene, Spata34 , encoding a LRR containing protein of 415 aa. Spata34 gene consisted of 9 exons and 8 introns and mapped to chromosome 3qA3. Spata34 is conserved across species in evolution. The Spata34 gene was expressed at various levels, faintly before first weeks postpartum and strongly from 2 weeks postpartum in adult testes. Western blot analysis showed that Spata34 protein was specially expressed in mouse testis. Immunohistochemical analysis revealed that Spata34 protein was most abundant in the cytoplasm of round spermatids and elongating spermatids within seminiferous tubules of the adult testis. Overexpression of Spata34 in COS7 cells inhibited the transcriptional activity of AP-1, p53 and p21 which suggested that Spata34 protein may act as a transcriptional repressor in p53 and p21 pathway.
ISSN:0301-4851
1573-4978
DOI:10.1007/s11033-013-3017-1