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Expression and identification of a novel gene Spata34 in mouse spermatogenic cells
Leucine-rich repeat (LRR) containing proteins play an essential role in signal transduction, cell adhesion, cell development, DNA repair and RNA processing. Here we cloned a novel gene, Spata34 , encoding a LRR containing protein of 415 aa. Spata34 gene consisted of 9 exons and 8 introns and mapped...
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| Published in: | Molecular biology reports 2014-03, Vol.41 (3), p.1683-1691 |
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| Main Authors: | , , , , , , , , , , , |
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| cites | cdi_FETCH-LOGICAL-c405t-11c4e17338a154024cd08be3f183536bc13614912a57ae9870d5b4eb41c2a0023 |
| container_end_page | 1691 |
| container_issue | 3 |
| container_start_page | 1683 |
| container_title | Molecular biology reports |
| container_volume | 41 |
| creator | Chen, Tingfang Jiang, Zhigang Xu, Wei Wang, Yuequn Li, Yongqing Wan, Yongqi Yuan, Wuzhou Mo, Xiaoyang Wu, Xiushan Deng, Yun Fan, Xiongwei Nie, Dongsong |
| description | Leucine-rich repeat (LRR) containing proteins play an essential role in signal transduction, cell adhesion, cell development, DNA repair and RNA processing. Here we cloned a novel gene,
Spata34
, encoding a LRR containing protein of 415 aa.
Spata34
gene consisted of 9 exons and 8 introns and mapped to chromosome 3qA3. Spata34 is conserved across species in evolution. The
Spata34
gene was expressed at various levels, faintly before first weeks postpartum and strongly from 2 weeks postpartum in adult testes. Western blot analysis showed that Spata34 protein was specially expressed in mouse testis. Immunohistochemical analysis revealed that Spata34 protein was most abundant in the cytoplasm of round spermatids and elongating spermatids within seminiferous tubules of the adult testis. Overexpression of Spata34 in COS7 cells inhibited the transcriptional activity of AP-1, p53 and p21 which suggested that Spata34 protein may act as a transcriptional repressor in p53 and p21 pathway. |
| doi_str_mv | 10.1007/s11033-013-3017-1 |
| format | article |
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Spata34
, encoding a LRR containing protein of 415 aa.
Spata34
gene consisted of 9 exons and 8 introns and mapped to chromosome 3qA3. Spata34 is conserved across species in evolution. The
Spata34
gene was expressed at various levels, faintly before first weeks postpartum and strongly from 2 weeks postpartum in adult testes. Western blot analysis showed that Spata34 protein was specially expressed in mouse testis. Immunohistochemical analysis revealed that Spata34 protein was most abundant in the cytoplasm of round spermatids and elongating spermatids within seminiferous tubules of the adult testis. Overexpression of Spata34 in COS7 cells inhibited the transcriptional activity of AP-1, p53 and p21 which suggested that Spata34 protein may act as a transcriptional repressor in p53 and p21 pathway.</description><identifier>ISSN: 0301-4851</identifier><identifier>EISSN: 1573-4978</identifier><identifier>DOI: 10.1007/s11033-013-3017-1</identifier><identifier>PMID: 24435972</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Amino Acid Sequence ; Animal Anatomy ; Animal Biochemistry ; Animals ; Biomedical and Life Sciences ; Cercopithecus aethiops ; Cloning ; COS Cells ; Female ; Gene expression ; Gene Expression Regulation, Developmental ; Germ Cells - growth & development ; Germ Cells - metabolism ; Histology ; Humans ; Life Sciences ; Male ; Membrane Proteins - biosynthesis ; Membrane Proteins - genetics ; Mice ; Molecular biology ; Morphology ; Repressor Proteins - genetics ; Rodents ; Sperm ; Spermatids - growth & development ; Spermatids - metabolism ; Spermatogenesis - genetics ; Testis - growth & development ; Testis - metabolism ; Transcription Factor AP-1 - biosynthesis ; Transcription Factor AP-1 - metabolism ; Tumor Suppressor Protein p53 - biosynthesis ; Tumor Suppressor Protein p53 - metabolism</subject><ispartof>Molecular biology reports, 2014-03, Vol.41 (3), p.1683-1691</ispartof><rights>Springer Science+Business Media Dordrecht 2014</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c405t-11c4e17338a154024cd08be3f183536bc13614912a57ae9870d5b4eb41c2a0023</citedby><cites>FETCH-LOGICAL-c405t-11c4e17338a154024cd08be3f183536bc13614912a57ae9870d5b4eb41c2a0023</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24435972$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chen, Tingfang</creatorcontrib><creatorcontrib>Jiang, Zhigang</creatorcontrib><creatorcontrib>Xu, Wei</creatorcontrib><creatorcontrib>Wang, Yuequn</creatorcontrib><creatorcontrib>Li, Yongqing</creatorcontrib><creatorcontrib>Wan, Yongqi</creatorcontrib><creatorcontrib>Yuan, Wuzhou</creatorcontrib><creatorcontrib>Mo, Xiaoyang</creatorcontrib><creatorcontrib>Wu, Xiushan</creatorcontrib><creatorcontrib>Deng, Yun</creatorcontrib><creatorcontrib>Fan, Xiongwei</creatorcontrib><creatorcontrib>Nie, Dongsong</creatorcontrib><title>Expression and identification of a novel gene Spata34 in mouse spermatogenic cells</title><title>Molecular biology reports</title><addtitle>Mol Biol Rep</addtitle><addtitle>Mol Biol Rep</addtitle><description>Leucine-rich repeat (LRR) containing proteins play an essential role in signal transduction, cell adhesion, cell development, DNA repair and RNA processing. Here we cloned a novel gene,
Spata34
, encoding a LRR containing protein of 415 aa.
Spata34
gene consisted of 9 exons and 8 introns and mapped to chromosome 3qA3. Spata34 is conserved across species in evolution. The
Spata34
gene was expressed at various levels, faintly before first weeks postpartum and strongly from 2 weeks postpartum in adult testes. Western blot analysis showed that Spata34 protein was specially expressed in mouse testis. Immunohistochemical analysis revealed that Spata34 protein was most abundant in the cytoplasm of round spermatids and elongating spermatids within seminiferous tubules of the adult testis. Overexpression of Spata34 in COS7 cells inhibited the transcriptional activity of AP-1, p53 and p21 which suggested that Spata34 protein may act as a transcriptional repressor in p53 and p21 pathway.</description><subject>Amino Acid Sequence</subject><subject>Animal Anatomy</subject><subject>Animal Biochemistry</subject><subject>Animals</subject><subject>Biomedical and Life Sciences</subject><subject>Cercopithecus aethiops</subject><subject>Cloning</subject><subject>COS Cells</subject><subject>Female</subject><subject>Gene expression</subject><subject>Gene Expression Regulation, Developmental</subject><subject>Germ Cells - growth & development</subject><subject>Germ Cells - metabolism</subject><subject>Histology</subject><subject>Humans</subject><subject>Life Sciences</subject><subject>Male</subject><subject>Membrane Proteins - biosynthesis</subject><subject>Membrane Proteins - genetics</subject><subject>Mice</subject><subject>Molecular biology</subject><subject>Morphology</subject><subject>Repressor Proteins - genetics</subject><subject>Rodents</subject><subject>Sperm</subject><subject>Spermatids - growth & development</subject><subject>Spermatids - metabolism</subject><subject>Spermatogenesis - genetics</subject><subject>Testis - growth & development</subject><subject>Testis - metabolism</subject><subject>Transcription Factor AP-1 - biosynthesis</subject><subject>Transcription Factor AP-1 - metabolism</subject><subject>Tumor Suppressor Protein p53 - biosynthesis</subject><subject>Tumor Suppressor Protein p53 - metabolism</subject><issn>0301-4851</issn><issn>1573-4978</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><recordid>eNqNkUtL5UAQhRtx0OvjB7iRBjduMlPVj3TuUsQXCAMz47rpdCoSuenE7lzRfz8drooIgquCqq9OneIwdoTwEwHMr4QIUhaAspCApsAttkBtZKGWptpmC8jdQlUad9leSg8AoNDoHbYrlJJ6acSC_bl4HiOl1A2Bu9DwrqEwdW3n3TS3hpY7HoYnWvF7CsT_jm5yUvEu8H5YJ-JppNi7acjTznNPq1U6YD9at0p0-Fr32d3lxb_z6-L299XN-dlt4RXoqUD0itBIWTnUCoTyDVQ1yRYrqWVZe5QlqiUKp42jZWWg0bWiWqEXDkDIfXa60R3j8LimNNm-S7MDFyh7s6g1lkJkrW-gWU-UIGb05BP6MKxjyI_MVHZaSmMyhRvKxyGlSK0dY9e7-GIR7JyN3WRjczZ2zsZi3jl-VV7XPTXvG29hZEBsgJRH4Z7ih9Nfqv4H71GWVg</recordid><startdate>20140301</startdate><enddate>20140301</enddate><creator>Chen, Tingfang</creator><creator>Jiang, Zhigang</creator><creator>Xu, Wei</creator><creator>Wang, Yuequn</creator><creator>Li, Yongqing</creator><creator>Wan, Yongqi</creator><creator>Yuan, Wuzhou</creator><creator>Mo, Xiaoyang</creator><creator>Wu, Xiushan</creator><creator>Deng, Yun</creator><creator>Fan, Xiongwei</creator><creator>Nie, Dongsong</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7TK</scope><scope>7TM</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20140301</creationdate><title>Expression and identification of a novel gene Spata34 in mouse spermatogenic cells</title><author>Chen, Tingfang ; Jiang, Zhigang ; Xu, Wei ; Wang, Yuequn ; Li, Yongqing ; Wan, Yongqi ; Yuan, Wuzhou ; Mo, Xiaoyang ; Wu, Xiushan ; Deng, Yun ; Fan, Xiongwei ; Nie, Dongsong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c405t-11c4e17338a154024cd08be3f183536bc13614912a57ae9870d5b4eb41c2a0023</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Amino Acid Sequence</topic><topic>Animal Anatomy</topic><topic>Animal Biochemistry</topic><topic>Animals</topic><topic>Biomedical and Life Sciences</topic><topic>Cercopithecus aethiops</topic><topic>Cloning</topic><topic>COS Cells</topic><topic>Female</topic><topic>Gene expression</topic><topic>Gene Expression Regulation, Developmental</topic><topic>Germ Cells - growth & development</topic><topic>Germ Cells - metabolism</topic><topic>Histology</topic><topic>Humans</topic><topic>Life Sciences</topic><topic>Male</topic><topic>Membrane Proteins - biosynthesis</topic><topic>Membrane Proteins - genetics</topic><topic>Mice</topic><topic>Molecular biology</topic><topic>Morphology</topic><topic>Repressor Proteins - genetics</topic><topic>Rodents</topic><topic>Sperm</topic><topic>Spermatids - growth & development</topic><topic>Spermatids - metabolism</topic><topic>Spermatogenesis - genetics</topic><topic>Testis - growth & development</topic><topic>Testis - metabolism</topic><topic>Transcription Factor AP-1 - biosynthesis</topic><topic>Transcription Factor AP-1 - metabolism</topic><topic>Tumor Suppressor Protein p53 - biosynthesis</topic><topic>Tumor Suppressor Protein p53 - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chen, Tingfang</creatorcontrib><creatorcontrib>Jiang, Zhigang</creatorcontrib><creatorcontrib>Xu, Wei</creatorcontrib><creatorcontrib>Wang, Yuequn</creatorcontrib><creatorcontrib>Li, Yongqing</creatorcontrib><creatorcontrib>Wan, Yongqi</creatorcontrib><creatorcontrib>Yuan, Wuzhou</creatorcontrib><creatorcontrib>Mo, Xiaoyang</creatorcontrib><creatorcontrib>Wu, Xiushan</creatorcontrib><creatorcontrib>Deng, Yun</creatorcontrib><creatorcontrib>Fan, Xiongwei</creatorcontrib><creatorcontrib>Nie, Dongsong</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Health & Medical Collection (Proquest)</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>PML(ProQuest Medical Library)</collection><collection>ProQuest Science Journals</collection><collection>ProQuest Biological Science Journals</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular biology reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chen, Tingfang</au><au>Jiang, Zhigang</au><au>Xu, Wei</au><au>Wang, Yuequn</au><au>Li, Yongqing</au><au>Wan, Yongqi</au><au>Yuan, Wuzhou</au><au>Mo, Xiaoyang</au><au>Wu, Xiushan</au><au>Deng, Yun</au><au>Fan, Xiongwei</au><au>Nie, Dongsong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression and identification of a novel gene Spata34 in mouse spermatogenic cells</atitle><jtitle>Molecular biology reports</jtitle><stitle>Mol Biol Rep</stitle><addtitle>Mol Biol Rep</addtitle><date>2014-03-01</date><risdate>2014</risdate><volume>41</volume><issue>3</issue><spage>1683</spage><epage>1691</epage><pages>1683-1691</pages><issn>0301-4851</issn><eissn>1573-4978</eissn><abstract>Leucine-rich repeat (LRR) containing proteins play an essential role in signal transduction, cell adhesion, cell development, DNA repair and RNA processing. Here we cloned a novel gene,
Spata34
, encoding a LRR containing protein of 415 aa.
Spata34
gene consisted of 9 exons and 8 introns and mapped to chromosome 3qA3. Spata34 is conserved across species in evolution. The
Spata34
gene was expressed at various levels, faintly before first weeks postpartum and strongly from 2 weeks postpartum in adult testes. Western blot analysis showed that Spata34 protein was specially expressed in mouse testis. Immunohistochemical analysis revealed that Spata34 protein was most abundant in the cytoplasm of round spermatids and elongating spermatids within seminiferous tubules of the adult testis. Overexpression of Spata34 in COS7 cells inhibited the transcriptional activity of AP-1, p53 and p21 which suggested that Spata34 protein may act as a transcriptional repressor in p53 and p21 pathway.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><pmid>24435972</pmid><doi>10.1007/s11033-013-3017-1</doi><tpages>9</tpages></addata></record> |
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| ispartof | Molecular biology reports, 2014-03, Vol.41 (3), p.1683-1691 |
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| source | Springer Nature |
| subjects | Amino Acid Sequence Animal Anatomy Animal Biochemistry Animals Biomedical and Life Sciences Cercopithecus aethiops Cloning COS Cells Female Gene expression Gene Expression Regulation, Developmental Germ Cells - growth & development Germ Cells - metabolism Histology Humans Life Sciences Male Membrane Proteins - biosynthesis Membrane Proteins - genetics Mice Molecular biology Morphology Repressor Proteins - genetics Rodents Sperm Spermatids - growth & development Spermatids - metabolism Spermatogenesis - genetics Testis - growth & development Testis - metabolism Transcription Factor AP-1 - biosynthesis Transcription Factor AP-1 - metabolism Tumor Suppressor Protein p53 - biosynthesis Tumor Suppressor Protein p53 - metabolism |
| title | Expression and identification of a novel gene Spata34 in mouse spermatogenic cells |
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