Genome-wide analyses of amphioxus microRNAs reveal an immune regulation via miR-92d targeting C3

Recently, amphioxus has served as a model for studying the origin and evolution of vertebrate immunity. However, little is known about how microRNAs (miRNAs) are involved in the immune defense in amphioxus. In this article, we present a systematic study of amphioxus miRNAs in the acute-phase respons...

Full description

Saved in:
Bibliographic Details
Published in:The Journal of immunology (1950) 2013-02, Vol.190 (4), p.1491-1500
Main Authors: Yang, Rirong, Zheng, Tingting, Cai, Xingsheng, Yu, Yingcai, Yu, Cuiling, Guo, Lei, Huang, Shengfeng, Zhu, Wei, Zhu, Ruimin, Yan, Qingyu, Ren, Zhenghua, Chen, Shangwu, Xu, Anlong
Format: Article
Language:English
Subjects:
Adaptive Immunity - genetics
Animals
Branchiostoma belcheri
Branchiostoma lanceolatum
Chordata
Chordata, Nonvertebrate - genetics
Chordata, Nonvertebrate - immunology
Chordata, Nonvertebrate - microbiology
Complement C3 - genetics
Complement C3 - metabolism
Disease Models, Animal
Evolution, Molecular
Gene Targeting - methods
Genome-Wide Association Study - methods
HEK293 Cells
Humans
MicroRNAs - biosynthesis
MicroRNAs - genetics
MicroRNAs - metabolism
Staphylococcal Infections - genetics
Staphylococcal Infections - immunology
Staphylococcus aureus
Vibrio anguillarum
Vibrio Infections - genetics
Vibrio Infections - immunology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Recently, amphioxus has served as a model for studying the origin and evolution of vertebrate immunity. However, little is known about how microRNAs (miRNAs) are involved in the immune defense in amphioxus. In this article, we present a systematic study of amphioxus miRNAs in the acute-phase response to bacterial infection; miR-92d was found to regulate the complement pathway in this basal chordate. We identified all 155 possible miRNAs present in the amphioxus Branchiostoma belcheri genome by bioinformatics analyses, including 57 newly identified miRNAs (called bbe-miRNAs), and characterized the miRNA expression pattern. Four miRNAs (bbe-miR-7, bbe-miR-4868a, bbe-miR-2065, and bbe-miR-34b) were upregulated and bbe-miR-92d was downregulated under the challenge of both Vibrio anguillarum and Staphylococcus aureus bacteria. We further predicted miRNA targets and identified mRNA targets of immune-related miRNA using the hybrid PCR method. We propose that miR-92d regulates the complement pathway through targeting C3 for controlling the acute immune response to bacterial infections. This study provides evidence for the complex immune regulation of miRNAs in the acute-phase response in basal chordates.
ISSN:0022-1767
1550-6606
DOI:10.4049/jimmunol.1200801