Vinculin arrests motile B cells by stabilizing integrin clustering at the immune synapse

Lymphocytes use integrin-based platforms to move and adhere firmly to the surface of other cells. The molecular mechanisms governing lymphocyte adhesion dynamics are however poorly understood. In this study, we show that in mouse B lymphocytes, the actin binding protein vinculin localizes to the rin...

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Bibliographic Details
Published in:The Journal of immunology (1950) 2013-09, Vol.191 (5), p.2742-2751
Main Authors: Saez de Guinoa, Julia, Barrio, Laura, Carrasco, Yolanda R
Format: Article
Language:English
Subjects:
Animals
B-Lymphocytes - immunology
B-Lymphocytes - metabolism
Blotting, Western
Cell Adhesion - immunology
Chemotaxis, Leukocyte - physiology
Fluorescent Antibody Technique
Immunological Synapses - immunology
Immunological Synapses - metabolism
Integrins - immunology
Integrins - metabolism
Lymphocyte Activation - physiology
Mice
Mice, Inbred C57BL
Mice, Transgenic
Vinculin - immunology
Vinculin - metabolism
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Summary:Lymphocytes use integrin-based platforms to move and adhere firmly to the surface of other cells. The molecular mechanisms governing lymphocyte adhesion dynamics are however poorly understood. In this study, we show that in mouse B lymphocytes, the actin binding protein vinculin localizes to the ring-shaped integrin-rich domain of the immune synapse (IS); the assembly of this platform, triggered by cognate immune interactions, is needed for chemokine-mediated B cell motility arrest and leads to firm, long-lasting B cell adhesion to the APC. Vinculin is recruited early in IS formation, in parallel to a local phosphatidylinositol (4,5)-bisphosphate wave, and requires spleen tyrosine kinase activity. Lack of vinculin at the IS impairs firm adhesion, promoting, in turn, cell migration with Ag clustered at the uropod. Vinculin localization to the B cell contact area depends on actomyosin. These results identify vinculin as a major controller of integrin-mediated adhesion dynamics in B cells.
ISSN:0022-1767
1550-6606
DOI:10.4049/jimmunol.1300684