Solution structure of a DNA mimicking motif of an RNA aptamer against transcription factor AML1 Runt domain

• Published in: Journal of biochemistry (Tokyo) 2013-12, Vol.154 (6), p.513-519
• Main Authors: Nomura, Yusuke, Tanaka, Yoichiro, Fukunaga, Jun-ichi, Fujiwara, Kazuya, Chiba, Manabu, Iibuchi, Hiroaki, Tanaka, Taku, Nakamura, Yoshikazu, Kawai, Gota, Kozu, Tomoko, Sakamoto, Taiichi
• Format: Article
• Language: English
• Subjects: Aptamers, Nucleotide - chemistry; Core Binding Factor Alpha 2 Subunit - antagonists & inhibitors; Core Binding Factor Alpha 2 Subunit - chemistry; DNA - chemistry; Humans; Molecular Mimicry; Nuclear Magnetic Resonance, Biomolecular; Nucleotide Motifs; Protein Structure, Tertiary; Solutions
• ISSN: 0021-924X; 1756-2651
• DOI: 10.1093/jb/mvt082

AML1/RUNX1 is an essential transcription factor involved in the differentiation of hematopoietic cells. AML1 binds to the Runt-binding double-stranded DNA element (RDE) of target genes through its N-terminal Runt domain. In a previous study, we obtained RNA aptamers against the AML1 Runt domain by systematic evolution of ligands by exponential enrichment and revealed that RNA aptamers exhibit higher affinity for the Runt domain than that for RDE and possess the 5'-GCGMGNN-3' and 5'-N'N'CCAC-3' conserved motif (M: A or C; N and N' form Watson-Crick base pairs) that is important for Runt domain binding. In this study, to understand the structural basis of recognition of the Runt domain by the aptamer motif, the solution structure of a 22-mer RNA was determined using nuclear magnetic resonance. The motif contains the AH(+)-C mismatch and base triple and adopts an unusual backbone structure. Structural analysis of the aptamer motif indicated that the aptamer binds to the Runt domain by mimicking the RDE sequence and structure. Our data should enhance the understanding of the structural basis of DNA mimicry by RNA molecules.