A novel peptide carrier for efficient targeting of antigens and nucleic acids to dendritic cells

Dendritic cells (DCs) initiate host immune responses by presenting captured antigens to naive T cells. Hence, DC‐binding peptides may be used for antigen targeting to boost naive and memory immune responses. By biopanning peptide phage libraries on human monocyte‐derived DCs, we identified novel DC‐...

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Bibliographic Details
Published in:The FASEB journal 2013-08, Vol.27 (8), p.3272-3283
Main Authors: Sioud, Mouldy, Skorstad, Gjertrud, Mobergslien, Anne, Sæb⊘e‐Larssen, Stein
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Antigen Presentation - immunology
Antigens - immunology
Antigens - metabolism
Binding, Competitive - immunology
cancer vaccines
Carrier Proteins - immunology
Carrier Proteins - metabolism
Cells, Cultured
cross presentation
Dendritic Cells - immunology
Dendritic Cells - metabolism
Flow Cytometry
HLA-A2 Antigen - immunology
HLA-A2 Antigen - metabolism
Humans
Leukocytes, Mononuclear - immunology
Leukocytes, Mononuclear - metabolism
Lymphocyte Activation - immunology
Microscopy, Fluorescence
Monocytes - immunology
Monocytes - metabolism
Nucleic Acids - immunology
Nucleic Acids - metabolism
Oligopeptides - immunology
Oligopeptides - metabolism
phage display
Phosphoproteins - immunology
Phosphoproteins - metabolism
Protein Binding - immunology
RNA, Small Interfering - genetics
RNA, Small Interfering - immunology
RNA, Small Interfering - metabolism
siRNA
T-Lymphocytes - immunology
T-Lymphocytes - metabolism
Viral Matrix Proteins - immunology
Viral Matrix Proteins - metabolism
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Description
Summary:Dendritic cells (DCs) initiate host immune responses by presenting captured antigens to naive T cells. Hence, DC‐binding peptides may be used for antigen targeting to boost naive and memory immune responses. By biopanning peptide phage libraries on human monocyte‐derived DCs, we identified novel DC‐binding peptides. One of the selected phages, displaying the NW peptide (NWYLPWLGTNDW), bound DCs with high affinity, and its binding was inhibited by the corresponding synthetic peptide. Antigenic peptides or proteins conjugated to the NW peptide bound to DCs and were internalized without negative effects on DC phenotype and function. Ex vivo targeted delivery of CMV‐pp65 peptides to DCs via the NW peptide increased T‐cell responses in HLA‐A2+/CMV+ donors compared to untargeted peptides (P
ISSN:0892-6638
1530-6860
DOI:10.1096/fj.12-224758