Enterobacter cloacae with a novel variant of ACT AmpC beta-lactamase originating from glaucous gull (Larus hyperboreus) in Svalbard

We aimed at Escherichia coli and Enterobacter cloacae isolates resistant to cephalosporins and fluoroquinolones and Salmonella isolates in wild birds in Arctic Svalbard, Norway. Cloacal swabs of little auks (Alle alle, n=215) and samples of faeces of glaucous gulls (Larus hyperboreus, n=15) were exa...

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Bibliographic Details
Published in:Veterinary microbiology 2014-07, Vol.171 (3-4), p.432-435
Main Authors: Literak, Ivan, Manga, Ivan, Wojczulanis-Jakubas, Katarzyna, Chroma, Magdalena, Jamborova, Ivana, Dobiasova, Hana, Sedlakova, Miroslava Htoutou, Cizek, Alois
Format: Article
Language:English
Subjects:
Alle alle
Amino Acid Substitution - genetics
Animals
Antimicrobial resistance
Bacteria
Bacterial Proteins - genetics
Bacterial Proteins - metabolism
Base Sequence
beta-Lactamases - genetics
beta-Lactamases - metabolism
Birds
Charadriiformes - microbiology
Cloaca - microbiology
DNA Primers - genetics
Drug Resistance, Bacterial - genetics
Enterobacter cloacae
Enterobacter cloacae - enzymology
Enterobacter cloacae - genetics
Escherichia coli
Escherichia coli - genetics
Larus hyperboreus
Molecular Sequence Data
Norway
Polymerase Chain Reaction - veterinary
Polymorphism, Single Nucleotide - genetics
Salmonella
Sequence Analysis, DNA - veterinary
Svalbard
Wildlife
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Summary:We aimed at Escherichia coli and Enterobacter cloacae isolates resistant to cephalosporins and fluoroquinolones and Salmonella isolates in wild birds in Arctic Svalbard, Norway. Cloacal swabs of little auks (Alle alle, n=215) and samples of faeces of glaucous gulls (Larus hyperboreus, n=15) were examined. Inducible production of AmpC enzyme was detected in E. cloacae KW218 isolate. Sequence analysis of the 1146bp PCR product of the ampC gene from this isolate revealed 99% sequence homology with the blaACT-14 and blaACT-5 AmpC beta-lactamase genes. Four, respectively six of the identified single nucleotide polymorphisms generated amino acid substitutions in the amino acid chain. As the ampC sequence polymorphism in the investigated E. cloacae strain was identified as unique, we revealed a novel variant of the ampC beta-lactamase gene blaACT-23.
ISSN:0378-1135
1873-2542
DOI:10.1016/j.vetmic.2014.02.015