Limitations of Pulsed‐Field Gel Electrophoresis for Analysis of Yersinia enterocolitica Isolates

This study describes the analysis of 432 isolates of Yersinia enterocolitica by pulsed‐field gel electrophoresis (PFGE). PFGE had a high level of discrimination with biotype 1A isolates (Simpson's Diversity Index 0.997), but with the clinically important biotypes 2, 3 and 4, the discriminatory...

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Bibliographic Details
Published in:Zoonoses and public health 2014-09, Vol.61 (6), p.405-410
Main Authors: Gilpin, B. J, Robson, B, Lin, S, Hudson, J. A, Weaver, L, Dufour, M, Strydom, H
Format: Article
Language:English
Subjects:
Bacterial Typing Techniques - methods
Biotype
biotypes
Disease Outbreaks
DNA, Bacterial - genetics
Electrophoresis, Gel, Pulsed-Field - methods
Epidemiology
gel electrophoresis
Genotype
Genotype & phenotype
genotyping
Humans
limitations
New Zealand
New Zealand - epidemiology
pulsed-field gel electrophoresis (PFGE)
Yersinia enterocolitica
Yersinia enterocolitica - genetics
Yersinia enterocolitica - isolation & purification
Yersinia Infections - epidemiology
Yersinia Infections - microbiology
yersiniosis
Zoonoses
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Summary:This study describes the analysis of 432 isolates of Yersinia enterocolitica by pulsed‐field gel electrophoresis (PFGE). PFGE had a high level of discrimination with biotype 1A isolates (Simpson's Diversity Index 0.997), but with the clinically important biotypes 2, 3 and 4, the discriminatory ability of PFGE was so low as to severely limit its usefulness (DI
ISSN:1863-1959
1863-2378
DOI:10.1111/zph.12085