Comparison of serum treatments to remove nonspecific inhibitors from chicken sera for the hemagglutination inhibition test with inactivated H5N1 and H9N2 avian Influenza A virus subtypes

The hemagglutination inhibition (HI) assay is the standard diagnostic test for detection of antibodies to avian influenza viruses. It is well known that chicken serum does not require additional serum pretreatment to remove nonspecific inhibitors (NSIs). However, NSIs were recognized in certain Kore...

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Bibliographic Details
Published in:Journal of veterinary diagnostic investigation 2012-09, Vol.24 (5), p.954-958
Main Authors: Kim, Hye-Ryoung, Lee, Kyoung-Ki, Kwon, Yong-Kuk, Kang, Min-Su, Moon, Oun-Kyung, Park, Choi-Kyu
Format: Article
Language:English
Subjects:
adsorption
Animals
antibodies
Antibodies, Viral - blood
avian influenza
blood
breeds
Chickens
Erythrocytes
heat treatment
hemagglutination
hemagglutination inhibition test
Hemagglutination Inhibition Tests - methods
Hemagglutination Inhibition Tests - veterinary
Hot Temperature
Influenza A virus
Influenza A Virus, H5N1 Subtype - isolation & purification
Influenza A Virus, H9N2 Subtype - isolation & purification
Influenza in Birds - diagnosis
Influenza in Birds - virology
Kaolin
serodiagnosis
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Summary:The hemagglutination inhibition (HI) assay is the standard diagnostic test for detection of antibodies to avian influenza viruses. It is well known that chicken serum does not require additional serum pretreatment to remove nonspecific inhibitors (NSIs). However, NSIs were recognized in certain Korean local breeds. In the present study, various treatments were compared to remove such NSIs. Heat treatment, red blood cell adsorption, and kaolin treatment did not remove NSIs effectively, and treatment with periodate only partly eliminated the NSIs. Receptor destroying enzyme (RDE) treatment appeared to effectively remove NSIs from chicken sera, regardless of breeds. It is proposed that RDE treatment should be included in the HI tests for serological diagnosis of avian Influenza A virus.
ISSN:1040-6387
1943-4936
DOI:10.1177/1040638712452732