Alterations in Neutrophil Production and Function at an Early Stage in the High-Fructose Rat Model of Metabolic Syndrome

• Published in: American journal of hypertension 2014-08, Vol.27 (8), p.1096-1104
• Main Authors: Tagzirt, Madjid, Corseaux, Delphine, Pasquesoone, Louise, Mouquet, Frédéric, Roma-Lavisse, Charlotte, Ung, Alexandre, Lorenzi, Rodrigo, Jude, Brigitte, Elkalioubie, Ahmed, Van Belle, Eric, Susen, Sophie, Dupont, Annabelle
• Format: Article
• Language: English
• Subjects: Abdominal Fat - pathology; Adipokines - blood; Animals; Apoptosis; Bone marrow; Bone Marrow - pathology; Cell Proliferation; Diet; Flow cytometry; Fructose; Granulocyte-Macrophage Colony-Stimulating Factor - blood; Granulocytes - pathology; Hypertension; Male; Metabolic syndrome; Metabolic Syndrome - blood; Metabolic Syndrome - chemically induced; Neutrophil Infiltration; Neutrophils; Rats; Rats, Sprague-Dawley; Reactive oxygen species; Rodents
• ISSN: 0895-7061; 1941-7225
• DOI: 10.1093/ajh/hpu021

BACKGROUND Although neutrophils are crucially involved in inflammation, they have received only little attention in metabolic syndrome (MetS). We hypothesized that neutrophil infiltration into adipose tissue (AT) may occur at an early stage of MetS, in association with modulation of major functions of neutrophils and of their bone marrow production. METHODS Fifty-six male Sprague-Dawley rats were fed regular (control rats (CRs)) or high-fructose (60%; fructose-fed rats (FFRs)) diets. After 6 weeks, metabolic parameters were measured. Distribution of neutrophils into AT was investigated by immunohistochemistry. Function of circulating neutrophils (activation, reactive oxygen species production, phagocytosis, and apoptosis) was determined by flow cytometry. Granulopoiesis was evaluated by measuring the number and survival characteristics of neutrophil progenitors using bone marrow culture assays and flow cytometry. RESULTS Compared with the CR group, the FFR group developed MetS (i.e., arterial hypertension, hypertriglyceridemia, fasting hyperglycemia, and greater intra-abdominal AT volume) and presented higher neutrophil infiltration into AT. At resting state, no significant difference for circulating neutrophil functions was observed between the 2 groups. In contrast, circulating neutrophils from the FFR group exhibited higher responses to phorbol-12-myristate-13-acetate for all studied functions, compared with the CR group, suggesting that early MetS induces neutrophil priming. In parallel, a diminished clonal capacity and an increased apoptosis in bone marrow-derived granulocyte progenitors and neutrophil precursors were observed in the FFR group compared with the CR group. CONCLUSIONS These results provide evidence of an increased infiltration into intra-abdominal AT and modified production, function, and phenotype of neutrophils at an early stage of high-fructose diet-induced MetS.