Immune response and disease resistance of carotenoids supplementation diet in Cyprinus carpio against Aeromonas hydrophila

• Published in: Fish & shellfish immunology 2014-09, Vol.40 (1), p.9-13
• Main Authors: Anbazahan, Sannasi Muthu, Shanthi Mari, Lourthu Samy, Yogeshwari, Govintharaj, Jagruthi, Chandrasekar, Thirumurugan, Ramasamy, Arockiaraj, Jesu, Joseph Velanganni, A. Antony, Krishnamoorthy, Palaniyandi, Balasundaram, Chellam, Harikrishnan, Ramasamy
• Format: Article
• Language: English
• Subjects: Aeromonas hydrophila; Aeromonas hydrophila - physiology; Animal Feed - analysis; Animals; Carotenoids; Carotenoids - pharmacology; Carps; Cyprinus carpio; Diet - veterinary; Dietary Supplements - analysis; Disease resistance; Disease Resistance - drug effects; Fish Diseases - immunology; Fish Diseases - microbiology; Gram-Negative Bacterial Infections - immunology; Gram-Negative Bacterial Infections - microbiology; Gram-Negative Bacterial Infections - veterinary; Immunity, Innate - drug effects; Innate immune response
• ISSN: 1050-4648; 1095-9947
• DOI: 10.1016/j.fsi.2014.06.011

The effect of carotenoid-supplementation diet on immune response and disease resistance in common carp, Cyprinus carpio against Aeromonas hydrophila at weeks 1, 2, and 4 is reported. The cumulative mortality was 10% when fish were fed with 50 or 100 mg kg−1 supplementation diets while the un-supplementation diet treated group suffered 90% mortality against the pathogen. The phagocytic activity and complement activity significantly increased with 50 and 100 mg kg−1 diet groups from weeks 2 and 4 but not in other groups. The reactive oxygen species (ROS) production was significantly enhanced with 50 and 100 mg kg−1 diets from weeks 1 to 4 while the production of reactive nitrogen species (RNS) enhanced on weeks 2 and 4. The lysozyme activity significantly increased when fed with 50 and 100 mg kg−1 diets on weeks 2 and all supplementation diets on week 4. These results suggest that diet enriched with carotenoid pigment positively enhance the immune status and protects C. carpio from A. hydrophila infection. •Cumulative mortality was 10% in 50 or 100 mg kg−1 diets against the pathogen.•Phagocytic and complement activity increased in 50 and 100 mg kg−1 diet on weeks 2 and 4.•ROS production was significantly enhanced with 50 and 100 mg kg−1 diets from weeks 1 to 4.•Lysozyme activity significantly increased all supplementation diets on week 4.•Diet enriched with carotenoid pigment is positively enhancing the immune system.