Bioreducible Cross-Linked Nanoshell Enhances Gene Transfection of Polycation/DNA Polyplex in Vivo

In this study, we have prepared a self-cross-linking PEG-based branched polymer, which easily forms a bioreducible nanoshell around polyplexes of cationic polymer and DNA, simply via heating the polyplex dispersions in the presence of this self-cross-linking branched polymer. This nanoshell can prev...

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Bibliographic Details
Published in:Biomacromolecules 2014-08, Vol.15 (8), p.2907-2913
Main Authors: Piao, Ji-Gang, Ding, Sheng-Gang, Yang, Lu, Hong, Chun-Yan, You, Ye-Zi
Format: Article
Language:English
Subjects:
Animals
Applied sciences
Biological and medical sciences
DNA - chemistry
DNA - pharmacology
Exact sciences and technology
General pharmacology
Male
Medical sciences
Mice
Nanoshells - chemistry
Organic polymers
Pharmaceutical technology. Pharmaceutical industry
Pharmacology. Drug treatments
Physicochemistry of polymers
Polyethylene Glycols - chemistry
Polyethylene Glycols - pharmacology
Polymers with particular properties
Preparation, kinetics, thermodynamics, mechanism and catalysts
Static Electricity
Transfection - methods
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Summary:In this study, we have prepared a self-cross-linking PEG-based branched polymer, which easily forms a bioreducible nanoshell around polyplexes of cationic polymer and DNA, simply via heating the polyplex dispersions in the presence of this self-cross-linking branched polymer. This nanoshell can prevent the polyplex from dissociation and aggregation in physiological fluids without inhibiting the electrostatic interactions between the polymer and DNA. Furthermore, glutathione (GSH) can act as a stimulus to open the nanoshell after it has entered the cell. The polyplexes coated with the bioreducible nanoshell show an obvious enhancement in gene transfection in vivo compared with bare polyplexes.
ISSN:1525-7797
1526-4602
DOI:10.1021/bm500518u