The effect of intermittent pneumatic compression of legs on the levels of nitric oxide related species in blood and on arterial function in the arm

• Published in: Nitric oxide 2014-08, Vol.40, p.117-122
• Main Authors: Rifkind, Joseph M., Nagababu, Enika, Dobrosielski, Devon A., Salgado, Maria T., Lima, Michael, Ouyang, Pamela, Silber, Harry A.
• Format: Article
• Language: English
• Subjects: Adult; Arm - blood supply; Female; Flow mediated dilation; Humans; Intermittent pneumatic compression; Intermittent Pneumatic Compression Devices; Leg - blood supply; Low-flow mediated constriction; Male; Nitric oxide; Nitric Oxide - blood; Nitric Oxide - metabolism; Nitrite; Occlusion-mediated constriction; Pilot Projects; Young Adult
• ISSN: 1089-8603; 1089-8611
• DOI: 10.1016/j.niox.2014.06.007

•We investigated nonlocal effects of leg intermittent pneumatic compression (IPC).•Serum nitrite and red blood cell nitric oxide decreased with 1h of IPC.•Occlusion-mediated constriction (OMC) of the distal brachial artery was attenuated.•We hypothesize a transfer of NO-bioactivity from blood to hypoxic arm tissue.•We hypothesize that NO species are released under hypoxic stress to oppose OMC. Intermittent pneumatic compression (IPC) of legs exerts beneficial local vascular effects, possibly through local release of nitric oxide (NO). However, studies demonstrating systemic transport of nitrogen oxide species and release of NO prompt the question of whether IPC could also exert nonlocal effects. We tested whether IPC (1) affects systemic levels of nitrite, S-nitrosothiols and red blood cell (RBC) NO, and (2) exerts vasoactive effects in the brachial artery (BA), although this hypothesis-generating pilot study did not investigate cause and effect relationship between (1) and (2). In 10 healthy subjects, ages 24–39years, we measured plasma nitrite, plasma S-nitrosothiols and RBC-NO from venous blood samples drawn before and after IPC treatment. We also measured BA responses to 5min of upper arm occlusion at rest and during1h of leg IPC. There was a significant decrease in plasma nitrite (112±26nM to 90±15nM, p=0.0008) and RBC-NO (129±72nM to 102±41nM, p=0.02). Plasma S-nitrosothiols were unchanged (5.79±4.81nM to 6.27±5.79nM, p=0.3). BA occlusion-mediated constriction (OMC) was significantly attenuated with IPC treatment (−43±13% to −33±12%, p=0.003). High-flow mediated BA dilation was unchanged (13.3±9.4% to 11.5±7.2%, p=0.2). Plasma nitrite, RBC-NO, and BA OMC decreased with leg IPC. We hypothesize that this decrease in circulatory pool of plasma nitrite and RBC-NO may result from the transfer of their NO-bioactivity from blood to the hypoxic arm tissue, to be stored and released under hypoxic stress and oppose OMC. Future studies should investigate whether IPC-induced decreases in brachial OMC are caused by the changes in systemic NO activity, and whether leg IPC could benefit distant arterial function in systemic cardiovascular disease.