Molecular analysis of JC polyomavirus genotypes circulating among tribal populations of North-Eastern West Bengal, India

There is a resurgence of interest in the study of occurrence, genotype and pathogenic associations of human Polyomaviruses in recent years. In the present study, we have ascertained the presence of human Polyomavirus JC (JCV) in the urine and peripheral blood leukocytes of tribal populations, for th...

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Bibliographic Details
Published in:Polish journal of microbiology 2014, Vol.63 (2), p.191-201
Main Authors: Chattaraj, Sutanuka, Bhattacharjee, Soumen
Format: Article
Language:English
Subjects:
Adult
Aged
Base Sequence
DNA, Viral - genetics
Female
Genotype
Genotypes
Humans
India - ethnology
JC Virus - classification
JC Virus - genetics
JC Virus - isolation & purification
Male
Middle Aged
Molecular Sequence Data
Polyomavirus Infections - epidemiology
Polyomavirus Infections - ethnology
Polyomavirus Infections - virology
Population Groups
Sequence Analysis, DNA
Young Adult
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Summary:There is a resurgence of interest in the study of occurrence, genotype and pathogenic associations of human Polyomaviruses in recent years. In the present study, we have ascertained the presence of human Polyomavirus JC (JCV) in the urine and peripheral blood leukocytes of tribal populations, for the first time in the North-Eastern part of West Bengal State of India. We have also characterized the prevalent genotypes of the non-coding controlregions (NCCRs) of these natural isolates. The result suggests a high incidence of JCV reactivation in the populations assayed. Approximately 25% of the non-immunocompromized tribal men and women, tested positive based on polymerase chain reaction (PCR) analysis, and these results were further confirmed by sequencing of PCR products. Pairwise sequence comparison and alignment of the NCCR sequence of these Indian strains appeared to be comparable and related to the archetypal JCV (CY) and the Tibetan LH3 strains, with some alterations in few key positions. The sequence analyses were done with regard to transcription factor binding to DNA sequence elements of endemic JCV NCCRs.
ISSN:1733-1331
2544-4646
DOI:10.33073/pjm-2014-025