A New Source of (R)-Limonene and Rotundifolone from Leaves of Lippia pedunculosa (Verbenaceae) and their Trypanocidal Properties

Investigation by GC-FID and GC-MS of the essential oil (LPOE) from the leaves of Lippia pedunculosa revealed, as the major compounds, the monoterpenes rotundifolone (71.7%) and (R)-limonene (21.8%). These two compounds and the minor constituent piperitenone (1.2%) were also isolated from the leaves...

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Bibliographic Details
Published in:Natural product communications 2014-06, Vol.9 (6), p.737-739
Main Authors: Menezes, Leociley Rocha Alencar, Santos, Nilmara Nunes, Meira, Cássio Santana, dos Santos, Jamyle Andrade Ferreira, Guimarães, Elisalva Teixeira, Soares, Milena Botelho Pereira, Nepel, Angelita, Barison, Andersson, Costa, Emmanoel Vilaça
Format: Article
Language:English
Subjects:
Animals
Cyclohexenes - chemistry
Cyclohexenes - pharmacology
Lippia - chemistry
Macrophages, Peritoneal - parasitology
Mice
Mice, Inbred BALB C
Molecular Structure
Monoterpenes - chemistry
Monoterpenes - pharmacology
Oils, Volatile - chemistry
Oils, Volatile - pharmacology
Plant Leaves - chemistry
Plant Oils - chemistry
Plant Oils - pharmacology
Terpenes - chemistry
Terpenes - pharmacology
Trypanocidal Agents - chemistry
Trypanocidal Agents - pharmacology
Trypanosoma cruzi - drug effects
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Summary:Investigation by GC-FID and GC-MS of the essential oil (LPOE) from the leaves of Lippia pedunculosa revealed, as the major compounds, the monoterpenes rotundifolone (71.7%) and (R)-limonene (21.8%). These two compounds and the minor constituent piperitenone (1.2%) were also isolated from the leaves and identified by spectrometric analysis. LPOE and isolated compounds were evaluated for their trypanocidal activity against epimastigote and trypomastigote forms of Trypanosoma cruzi. Significant results with IC50 values lower than 34.0 μg.mL−1 were observed against these forms of T. cruzi for LPOE and isolated compounds. Rotundifolone was the most active compound with an IC50 lower than 10.0 μg.mL–1 for both forms of T. cruzi. The effects of LPOE and isolated compounds were also evaluated in cultures of macrophages infected with T. cruzi. Treatment with (R)-limonene and rotundifolone caused a moderate reduction in the percentage of macrophages infected by T. cruzi and in the number of intracellular parasites at concentrations non-toxic to macrophages.
ISSN:1934-578X
1555-9475
DOI:10.1177/1934578X1400900601