On-line multidimensional liquid chromatographic determination of polynuclear aromatic hydrocarbons in complex samples

A method is described for the on-line coupling of a normal-phase high-performance liquid chromtographic (HPLC) system to a reversed-phased HPLC system. The method employs a diamine column for on-column concentration of a selected fraction from a normal-phase aminosilane column followed by a solvent...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 1982-04, Vol.54 (4), p.723-727
Main Authors: Sonnefeld, W. J, Zoller, W. H, May, W. E, Wise, S. A
Format: Article
Language:English
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Summary:A method is described for the on-line coupling of a normal-phase high-performance liquid chromtographic (HPLC) system to a reversed-phased HPLC system. The method employs a diamine column for on-column concentration of a selected fraction from a normal-phase aminosilane column followed by a solvent exchange procedure and gradlent elution focusing of the analyte species onto a reversed-phase octadecylsilane column. No loss of analyte of chromatographic resolution is observed by using this method. Several chromatographic packing materials were investigated for use as on-column concentrators of polynuclear aromatic hydrocarbons (PAH) from normal-phase chromatographic systems. The valldity of this approach was verified by determining the concentration of several PAH in Standard Reference Material (SRM) 1580 -- "Organics in Shale Oil".
ISSN:0003-2700
1520-6882
DOI:10.1021/ac00241a029